Oshima Tatsuya, Kanemaru Kenzo, Tachiyama Hodzumi, Ohe Kaoru, Baba Yoshinari
Department of Applied Chemistry, Faculty of Engineering, University of Miyazaki, 1-1 Gakuen Kibanadai Nishi, Miyazaki, Japan.
J Chromatogr B Analyt Technol Biomed Life Sci. 2008 Dec 1;876(1):116-22. doi: 10.1016/j.jchromb.2008.10.039. Epub 2008 Oct 30.
Adsorption of histidine-containing dipeptides such as carnosine (Car) was investigated using copper(II) immobilized cation exchange resins. Adsorption of Car was enhanced using Cu(II) immobilized resins, on the basis of metal affinity interactions. In particular, iminodiacetic acid chelating resin with immobilized Cu(II) (Cu-IDA) can adsorb Car from saline water. Car was adsorbed on Cu-IDA even in the presence of 1000 mM of NaCl. Adsorption of various amino acids on Cu-IDA was compared under same conditions. Histidine and the histidine-containing dipeptides were selectively adsorbed on Cu-IDA over other amino acids, both in the absence and in the presence of NaCl. Therefore, immobilized metal affinity adsorption is an efficient method for recovering histidine-containing dipeptides from saline water.
使用固定化铜（II）阳离子交换树脂研究了含组氨酸的二肽如肌肽（Car）的吸附。基于金属亲和相互作用，使用固定化铜（II）的树脂可增强肌肽的吸附。特别是，固定化铜（II）的亚氨基二乙酸螯合树脂（Cu-IDA）可以从盐水中吸附肌肽。即使在存在1000 mM氯化钠的情况下，肌肽也能被吸附在Cu-IDA上。在相同条件下比较了各种氨基酸在Cu-IDA上的吸附情况。在不存在和存在氯化钠的情况下，组氨酸和含组氨酸的二肽在Cu-IDA上比其他氨基酸更易被选择性吸附。因此，固定化金属亲和吸附是从盐水中回收含组氨酸二肽的有效方法。
