Lenka Sangram K, Lohia Bikash, Kumar Abhay, Chinnusamy Viswanathan, Bansal Kailash C
National Research Centre on Plant Biotechnology, Indian Agricultural Research Institute, New Delhi, 110012, India.
Plant Mol Biol. 2009 Feb;69(3):261-71. doi: 10.1007/s11103-008-9423-4. Epub 2008 Nov 8.
Abscisic acid (ABA), the popular plant stress hormone, plays a key role in regulation of sub-set of stress responsive genes. These genes respond to ABA through specific transcription factors which bind to cis-regulatory elements present in their promoters. We discovered the ABA Responsive Element (ABRE) core (ACGT) containing CGMCACGTGB motif as over-represented motif among the promoters of ABA responsive co-expressed genes in rice. Targeted gene prediction strategy using this motif led to the identification of 402 protein coding genes potentially regulated by ABA-dependent molecular genetic network. RT-PCR analysis of arbitrarily chosen 45 genes from the predicted 402 genes confirmed 80% accuracy of our prediction. Plant Gene Ontology (GO) analysis of ABA responsive genes showed enrichment of signal transduction and stress related genes among diverse functional categories.
脱落酸（ABA）是一种常见的植物应激激素，在调控应激反应基因子集方面发挥着关键作用。这些基因通过特定的转录因子对ABA作出反应，这些转录因子与它们启动子中存在的顺式调控元件结合。我们发现，在水稻中，ABA响应共表达基因的启动子中，含有CGMCACGTGB基序的ABA响应元件（ABRE）核心（ACGT）是过度呈现的基序。使用该基序的靶向基因预测策略导致鉴定出402个可能受ABA依赖分子遗传网络调控的蛋白质编码基因。对预测的402个基因中任意选择的45个基因进行RT-PCR分析，证实了我们预测的80%准确率。对ABA响应基因的植物基因本体（GO）分析表明，在不同功能类别中，信号转导和应激相关基因富集。
