Tran Lam-Son Phan, Nakashima Kazuo, Shinozaki Kazuo, Yamaguchi-Shinozaki Kazuko
Biological Resources Division, Japan International Research Center for Agricultural Sciences, Tsukuba, Ibaraki, Japan.
Methods Enzymol. 2007;428:109-28. doi: 10.1016/S0076-6879(07)28006-1.
Because of their sessile nature, plants grown in a dynamic climate have evolved a range of adaptations that enable them to survive in various environmental stress conditions during growth and development. Plants respond to environmental stresses at both cellular and molecular levels by altering the expression of many genes via a complexity of signaling pathways. These pathways begin with signal perception and end with the expression of stress-responsive target genes. Ultimately, the selective upregulation of target genes leads to the alteration of physiological response so as to confer tolerance of the stress. In the signal transduction network, various regulatory and functional proteins function collectively to ensure survival of the plants. This chapter summarizes the methodology used to dissect gene regulatory networks involved in the response to osmotic stresses, such as drought and high salinity.
由于其固着生长的特性，生长在动态气候条件下的植物进化出了一系列适应性机制，使其能够在生长发育过程中应对各种环境胁迫条件而存活下来。植物通过复杂的信号通路改变许多基因的表达，从而在细胞和分子水平上对环境胁迫做出反应。这些通路始于信号感知，终于胁迫响应靶基因的表达。最终，靶基因的选择性上调导致生理反应的改变，从而赋予植物对胁迫的耐受性。在信号转导网络中，各种调节蛋白和功能蛋白共同发挥作用，以确保植物的存活。本章总结了用于剖析参与渗透胁迫（如干旱和高盐度）响应的基因调控网络的方法。
