[实验性精索静脉曲张对大鼠血清和睾丸中睾酮水平的影响]
[Effects of experimental varicocele on the testosterone level in the serum and testis of rats].
作者信息
Liu Jian-jun, Dong Qiang, Yang Yu-ru
机构信息
Department of Urology, Peoples Hospital of Henan Province, Zhengzhou, Henan 450003, China.
出版信息
Zhonghua Nan Ke Xue. 2007 Apr;13(4):335-7.
OBJECTIVE
To study the effects of experimental varicocele (VC) on the serum testosterone (T) and intratesticular testosterone in adolescent rats.
METHODS
A VC rat model was established by partial ligation of the left kidney vein in 20 SD rats, and another 20 were included in a sham operation group as controls. At 4 and 8 weeks, the concentrations of the serum T and intratesticular T were measured by radioimmunoassay (RIA). The testis tissues were homogenized and the extract liquid taken for RIA.
RESULTS
Compared with the controls, the level of serum T declined at 4 and 8 weeks in the VC group, but not significantly (P > 0.05), so was that of bilateral intratesticular T at 4 weeks, and with statistical significance at 8 (P < 0.01).
CONCLUSION
Within 8 weeks, experimental VC could reduce the level of bilateral intratesticular T, but not that of serum T. Varicocele could damage Leydig cells.
目的
研究实验性精索静脉曲张（VC）对青春期大鼠血清睾酮（T）及睾丸内睾酮的影响。
方法
通过部分结扎20只SD大鼠的左肾静脉建立VC大鼠模型，另外20只作为假手术组作为对照。在4周和8周时，采用放射免疫分析法（RIA）测定血清T和睾丸内T的浓度。将睾丸组织匀浆并取提取液进行RIA检测。
结果
与对照组相比，VC组在4周和8周时血清T水平下降，但差异不显著（P>0.05），4周时双侧睾丸内T水平也是如此，8周时有统计学意义（P<0.01）。
结论
在8周内，实验性VC可降低双侧睾丸内T水平，但不影响血清T水平。精索静脉曲张可损害睾丸间质细胞。
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