Laperle Christopher M, Hamilton Theron J, Wintermeyer Philip, Walker Evan J, Shi Daxin, Anastasio Mark A, Derdak Zoltan, Wands Jack R, Diebold Gerald, Rose-Petruck Christoph
The Liver Research Center, Rhode Island Hospital and Warren Alpert Medical School of Brown University, Providence, RI, USA.
Phys Med Biol. 2008 Dec 7;53(23):6911-23. doi: 10.1088/0031-9155/53/23/017. Epub 2008 Nov 12.
We report a new preparative method for providing contrast through reduction in electron density that is uniquely suited for propagation-based differential x-ray phase contrast imaging. The method, which results in an air or fluid filled vasculature, makes possible visualization of the smallest microvessels, roughly down to 15 microm, in an excised murine liver, while preserving the tissue for subsequent histological workup. We show the utility of spatial frequency filtering for increasing the visibility of minute features characteristic of phase contrast imaging, and the capability of tomographic reconstruction to reveal microvessel structure and three-dimensional visualization of the sample. The effect of water evaporation from livers during x-ray imaging on the visibility of blood vessels is delineated. The deformed vascular tree in a cancerous murine liver is imaged.
我们报告了一种新的制备方法，该方法通过降低电子密度来提供对比度，这特别适用于基于传播的差分X射线相衬成像。该方法可形成充满空气或液体的脉管系统，能够在切除的小鼠肝脏中可视化最小约15微米的微血管，同时保留组织以便后续进行组织学检查。我们展示了空间频率滤波在提高相衬成像特征性微小特征可见性方面的效用，以及断层重建揭示微血管结构和样品三维可视化的能力。阐述了X射线成像过程中小鼠肝脏水分蒸发对血管可见性的影响。对癌性小鼠肝脏中变形的血管树进行了成像。
