Zysk Adam M, Garson Alfred B, Xu Qiaofeng, Brey Eric M, Zhou Wei, Brankov Jovan G, Wernick Miles N, Kuszak Jerome R, Anastasio Mark A
Biomed Opt Express. 2012 Aug 1;3(8):1924-32. doi: 10.1364/BOE.3.001924. Epub 2012 Jul 24.
The in vitro investigation of many optically opaque biological microstructures requires 3D analysis at high resolution over a large field of view. We demonstrate a new nondestructive volumetric imaging technique that eliminates the structural and computational limitations of conventional 2D optical microscopy by combining x-ray phase-contrast tomography with critical point drying sample preparation. We experimentally demonstrate the enhancement of small features afforded by phase-contrast imaging and show the contrast improvement afforded by the drying of a hydrated specimen. We further demonstrate the biological application of this technique by imaging the microstructure of the accommodative apparatus in a primate eye using a benchtop phase-contrast tomography system.
对许多光学不透明的生物微观结构进行体外研究需要在大视野范围内进行高分辨率的三维分析。我们展示了一种新的无损体积成像技术，该技术通过将X射线相衬断层扫描与临界点干燥样品制备相结合，消除了传统二维光学显微镜的结构和计算限制。我们通过实验证明了相衬成像对小特征的增强作用，并展示了水合标本干燥所带来的对比度改善。我们还使用台式相衬断层扫描系统对灵长类动物眼睛中调节装置的微观结构进行成像，进一步证明了该技术的生物学应用。
