Yu Longquan, Shirai Nobuya, Suzuki Hiramitsu, Hosono Tsuyoshi, Nakajima Yoshijiro, Kajiwara Masahiro, Takatori Kazuhiro
United Graduate School of Agricultural Science, Tokyo University of Agriculture and Technology, Tokyo, Japan.
J Nutr Sci Vitaminol (Tokyo). 2008 Oct;54(5):378-83. doi: 10.3177/jnsv.54.378.
Lipids extracted from tsao-ko were separated into three fractions with silica gel column chromatography and fed to mice (3 mo old) for 90 d to clarify their inhibitory activity on digestive enzyme activity. The diets contained the following: control--no tsao-ko, 0.05% total lipid of tsao-ko (TL), 0.0109% chloroform fraction (CF), 0.0245% acetone fraction (AF), or 0.00365% methanol fraction (MeF). Although CF and AF slightly inhibited the activities of alpha-glucosidase, alpha-amylase, and lipase, intakes of these fractions had little influence on plasma and liver lipid concentrations when compared with the control diet. MeF did not inhibit alpha-glucosidase but had DPPH radical scavenging activity and the mice fed this fraction had the most marked reduction in plasma glucose and TBARS concentrations compared with the other diet groups. These results suggest that the fat-soluble polar components of tsao-ko contain an active component that might be associated with decreased plasma glucose and TBARS concentrations in mice.
从草果中提取的脂质通过硅胶柱色谱法分离为三个组分，并喂食给3月龄小鼠90天，以阐明它们对消化酶活性的抑制作用。饮食包含以下几种：对照组——不含草果，0.05%草果总脂质（TL），0.0109%氯仿组分（CF），0.0245%丙酮组分（AF），或0.00365%甲醇组分（MeF）。尽管CF和AF对α-葡萄糖苷酶、α-淀粉酶和脂肪酶的活性有轻微抑制作用，但与对照饮食相比，这些组分的摄入量对血浆和肝脏脂质浓度影响很小。MeF不抑制α-葡萄糖苷酶，但具有DPPH自由基清除活性，与其他饮食组相比，喂食该组分的小鼠血浆葡萄糖和TBARS浓度降低最为显著。这些结果表明，草果的脂溶性极性成分含有一种活性成分，可能与小鼠血浆葡萄糖和TBARS浓度降低有关。
