de Sousa Ricardo Luiz Moro, Moreli Marcos Lázaro, Borges Alessandra Abel, Campos Gelse Mazzoni, Livonesi Márcia Cristina, Figueiredo Luiz Tadeu Moraes, Pinto Aramis Augusto
Department of Basic Sciences, School of Zootechny and Food Engineering, University of São Paulo, Pirassununga, SP, Brazil.
Intervirology. 2008;51(4):299-310. doi: 10.1159/000171818. Epub 2008 Nov 12.
Hantaviruses are rodent-borne RNA viruses that have caused hantavirus cardiopulmonary syndrome in several Brazilian regions. In the present study, geographical distribution, seroprevalence, natural host range, and phylogenetic relations of rodent-associated hantaviruses collected from seven counties of Southeastern Brazil were evaluated.
ELISA, RT-PCR and phylogenetic analysis were used in this study.
Antibodies to hantavirus were detected in Bolomys lasiurus, Akodon sp. and Oligoryzomys sp., performing an overall seroprevalence of 5.17%. All seropositive rodents were associated with grasslands or woods surrounded by sugar cane fields. Phylogenetic analysis of partial S- and M-segment sequences showed that viral sequences isolated from B. lasiurus specimens clustered with Araraquara virus. However, a sequence from Akodon sp. shared 100% similarity with Argentinian/Chilean viruses based on the partial S-segment amino acid sequence.
These results indicate that there are associations between rodent reservoirs and hantaviruses in some regions of Southeastern Brazil, and suggest the existence of additional hantavirus genetic diversity and host ecology in these areas.
汉坦病毒是由啮齿动物传播的RNA病毒，已在巴西多个地区引发汉坦病毒心肺综合征。在本研究中，对从巴西东南部七个县采集的与啮齿动物相关的汉坦病毒的地理分布、血清流行率、自然宿主范围和系统发育关系进行了评估。
本研究采用酶联免疫吸附测定（ELISA）、逆转录-聚合酶链反应（RT-PCR）和系统发育分析。
在巴西草原鹿鼠、阿氏稻鼠属和稻鼠属中检测到汉坦病毒抗体，总体血清流行率为5.17%。所有血清阳性的啮齿动物都与被甘蔗田包围的草原或树林有关。对部分S片段和M片段序列的系统发育分析表明，从巴西草原鹿鼠标本中分离出的病毒序列与阿拉拉夸拉病毒聚集在一起。然而，基于部分S片段氨基酸序列，来自阿氏稻鼠属的一个序列与阿根廷/智利病毒具有100%的相似性。
这些结果表明，在巴西东南部的一些地区，啮齿动物宿主与汉坦病毒之间存在关联，并表明这些地区存在其他汉坦病毒遗传多样性和宿主生态。
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