核小体不是通过微球菌核酸酶消化从四膜虫染色质中产生的。
Chromatosomes are not produced from Tetrahymena chromatin by micrococcal nuclease digestion.
作者信息
Suda M
机构信息
Institute of Endocrinology, Gunma University, Japan.
出版信息
Experientia. 1991 Jan 15;47(1):54-6. doi: 10.1007/BF02041251.
PMID:1900245
Abstract
The subnucleosomal organization of Tetrahymena chromatin, which has an unusual H1 histone, was investigated by NaCl extraction and micrococcal nuclease digestion of nuclei. It was found that Tetrahymena histone H1 is extracted with 0.35 M NaCl, whereas bovine thyroid H1 is not. Micrococcal nuclease digestion of Tetrahymena nuclei did not yield chromatosomes as a stable intermediate, whereas digestion of bovine thyroid nuclei did.
摘要
通过对细胞核进行氯化钠提取和微球菌核酸酶消化，研究了具有异常H1组蛋白的四膜虫染色质的亚核小体组织。结果发现，四膜虫组蛋白H1在0.35M氯化钠中被提取出来，而牛甲状腺H1则不会。对四膜虫细胞核进行微球菌核酸酶消化不会产生作为稳定中间体的核小体，而对牛甲状腺细胞核进行消化则会产生。
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