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核小体不是通过微球菌核酸酶消化从四膜虫染色质中产生的。

Chromatosomes are not produced from Tetrahymena chromatin by micrococcal nuclease digestion.

作者信息

Suda M

机构信息

Institute of Endocrinology, Gunma University, Japan.

出版信息

Experientia. 1991 Jan 15;47(1):54-6. doi: 10.1007/BF02041251.

DOI:10.1007/BF02041251
PMID:1900245
Abstract

The subnucleosomal organization of Tetrahymena chromatin, which has an unusual H1 histone, was investigated by NaCl extraction and micrococcal nuclease digestion of nuclei. It was found that Tetrahymena histone H1 is extracted with 0.35 M NaCl, whereas bovine thyroid H1 is not. Micrococcal nuclease digestion of Tetrahymena nuclei did not yield chromatosomes as a stable intermediate, whereas digestion of bovine thyroid nuclei did.

摘要

通过对细胞核进行氯化钠提取和微球菌核酸酶消化，研究了具有异常H1组蛋白的四膜虫染色质的亚核小体组织。结果发现，四膜虫组蛋白H1在0.35M氯化钠中被提取出来，而牛甲状腺H1则不会。对四膜虫细胞核进行微球菌核酸酶消化不会产生作为稳定中间体的核小体，而对牛甲状腺细胞核进行消化则会产生。

相似文献

1
Chromatosomes are not produced from Tetrahymena chromatin by micrococcal nuclease digestion.核小体不是通过微球菌核酸酶消化从四膜虫染色质中产生的。
Experientia. 1991 Jan 15;47(1):54-6. doi: 10.1007/BF02041251.
2
Histones and chromatin structure in Tetrahymena macro- and micronuclei.
Cold Spring Harb Symp Quant Biol. 1978;42 Pt 1:493-503. doi: 10.1101/sqb.1978.042.01.052.
3
Subunit structure of a naturally occurring chromatin lacking histones F1 and F3.缺乏组蛋白F1和F3的天然存在染色质的亚基结构。
Proc Natl Acad Sci U S A. 1975 Sep;72(9):3536-40. doi: 10.1073/pnas.72.9.3536.
4
Structure of rDNA-containing chromatin of Tetrahymena pyriformis analyzed by nuclease digestion.通过核酸酶消化分析梨形四膜虫含核糖体DNA染色质的结构
Cold Spring Harb Symp Quant Biol. 1978;42 Pt 2:773-8. doi: 10.1101/sqb.1978.042.01.077.
5
The effects of salt concentration and H-1 depletion on the digestion of calf thymus chromatin by micrococcal nuclease.盐浓度和H-1缺失对微球菌核酸酶消化小牛胸腺染色质的影响。
Nucleic Acids Res. 1979;6(5):1843-62. doi: 10.1093/nar/6.5.1843.
6
Histone H1 affects the loss of chromatin fragments from nuclease-treated nuclei.组蛋白H1影响经核酸酶处理的细胞核中染色质片段的丢失。
J Biol Chem. 1982 Jun 10;257(11):6576-80.
7
Distribution of H1 histone in chromatin digested by micrococcal nuclease.微球菌核酸酶消化染色质中H1组蛋白的分布
Nucleic Acids Res. 1977 Oct;4(10):3281-301. doi: 10.1093/nar/4.10.3281.
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Superstructural differences between chromatin in nuclei and in solution are revealed by kinetics of micrococcal nuclease digestion.
J Biol Chem. 1979 Oct 10;254(19):9477-87.
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Fractionation of nucleosomes by salt elution from micrococcal nuclease-digested nuclei.通过从微球菌核酸酶消化的细胞核中进行盐洗脱来分离核小体。
J Cell Biol. 1978 Oct;79(1):97-109. doi: 10.1083/jcb.79.1.97.
10
Anomalous nuclease digestion of Holothuria sperm chromatin containing histone H1 variants.
Eur J Biochem. 1985 May 2;148(3):529-32. doi: 10.1111/j.1432-1033.1985.tb08871.x.

本文引用的文献

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The structure of histone H1 and its location in chromatin.组蛋白H1的结构及其在染色质中的位置。
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Tetrahymena histone H1. Isolation and amino acid sequence lacking the central hydrophobic domain conserved in other H1 histones.四膜虫组蛋白H1。分离及氨基酸序列，其缺少在其他H1组蛋白中保守的中央疏水结构域。
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A protein that accumulates during starvation in Tetrahymena nuclei.一种在饥饿期间于四膜虫细胞核中积累的蛋白质。
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7
Studies on the role and mode of operation of the very-lysine-rich histone H1 in eukaryote chromatin. The three structural regions of the histone H1 molecule.富含赖氨酸的组蛋白H1在真核生物染色质中的作用及作用模式研究。组蛋白H1分子的三个结构区域。
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