da Cruz M Q, Bräscher H M, Vargens J R, Oliveira-Lima A
Instituto de Puericultura e Pediatria da UFRJ, Rio de Janeiro, Brazil.
Experientia. 1991 Jan 15;47(1):89-92. doi: 10.1007/BF02041263.
Viable metacyclic forms of T. cruzi, Y strain, treated with an adequate dose of actinomycin D (50 micrograms Act-D/ml/10(7) parasites/ml for 72 h at 28 degrees C) showed the following properties: 1) they lost their ability to replicate in culture medium, in blood and in tissues of normal mice and were no longer able to incorporate tritiated thymidine; 2) they could not penetrate into Vero cells and could not replicate inside normal macrophages; 3) they retained their immunogenicity and the ability to protect mice against a virulent infection; 4) they did not induce histological lesions as described in chronic experimental Chagas' disease.
用适量放线菌素D（50微克放线菌素D/毫升/每10⁷个寄生虫/毫升，于28℃处理72小时）处理过的克氏锥虫Y株有活力的循环后期型表现出以下特性：1）它们失去了在培养基、血液及正常小鼠组织中复制的能力，且不再能够掺入氚标记的胸腺嘧啶核苷；2）它们无法侵入Vero细胞，也不能在正常巨噬细胞内复制；3）它们保留了免疫原性以及保护小鼠抵御强毒感染的能力；4）它们不会诱发慢性实验性恰加斯病中所描述的组织学损伤。
