Department of Applied Biological Chemistry, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo, 113-8657, Japan.
Cytotechnology. 2000 Jul;33(1-3):53-61. doi: 10.1023/A:1008159211932.
In the mammalian central nervous system, a complexcircuit of neurons contributes to higher behaviors.Each region of the brain has a unique function derivedfrom various types of neurons. Several neuralprecursor cell lines have been established from basalganglia of fetal brain. In this study, hippocampalneural precursor cell lines were established from thehippocampus of p53(-/-) embryos. By means ofintegration of a MycER regulatable oncoprotein intop53(-/-) neural precursor cells, several immortallines were established from embryonic hippocampalprimordium, with bFGF and estrogen continuouslysupplied for activation of the MycER protein. A dualluciferase study demonstrated that the MycER proteinblocked the expression of a glial cell marker protein,GFAP, probably contributing to the persistent celldivision of the immortalized neural precursor cells.These cell lines differentiate into neuronal and glialcell types after withdrawal of bFGF. The phenotype ofthe hippocampal cell lines differed from that of thebasal ganglia cell lines as observed in a clonaldensity culture. This result implies that each regionof the brain has a unique developmental program, thatmay be imprinted in each of the neural precursor cells.
在哺乳动物中枢神经系统中，神经元的复杂回路有助于高级行为。大脑的每个区域都具有源自各种类型神经元的独特功能。已经从胎脑的基底神经节中建立了几种神经前体细胞系。在这项研究中，从 p53(-/-)胚胎的海马中建立了海马神经前体细胞系。通过将 MycER 可调控的致癌蛋白整合到 p53(-/-)神经前体细胞中，从小鼠胚胎海马原基中建立了几个永生化系，持续提供 bFGF 和雌激素以激活 MycER 蛋白。双荧光素酶研究表明，MycER 蛋白阻断了神经胶质细胞标记蛋白 GFAP 的表达，这可能有助于永生化神经前体细胞的持续细胞分裂。撤去 bFGF 后，这些细胞系可分化为神经元和神经胶质细胞。与在克隆密度培养中观察到的基底神经节细胞系相比，海马细胞系的表型不同。这一结果表明，大脑的每个区域都具有独特的发育程序，这些程序可能被印刻在每个神经前体细胞中。
