Department of Applied Biological Chemistry, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo, 113-8657, Japan.
Cytotechnology. 2000 Jul;33(1-3):157-65. doi: 10.1023/A:1008102621059.
Both neurons and glial cells are derived from neuralprecursor cells in the ventricular zone during braindevelopment. The fate of the neural precursor cells isaffected by neurotransmitters such as glutamate. Inthis study, we examined glutamate-triggeredintracellular Ca(2+) signaling in neural precursorcell lines by the calcium digital imaging method. Whenimmortalized primary-cultured neural precursor cellswere treated with glutamate, a subpopulation of thesecells showed an increase in intracellular Ca(2+)concentration. In an effort to determine the role ofthe glutamate-triggered intracellular Ca(2+) signalin neural precursor cells, we tried to cultureimmortalized basal ganglial and hippocampal neuralprecursor cell lines in glutamate-free medium. Thehippocampal (MHP-2) cells became adapted to theglutamate-free medium, and when treated with glutamatethe adapted subline (MHP-2-E1) showed an increase inintracellular Ca(2+) concentration. In contrast,the basal ganglial neural precursor cell lines failedto become adapted to the glutamate-free medium. Theseresults suggest that hippocampal and basal ganglialneural precursor cells differ in their cellularresponse to glutamate as an exogenous stimulus.
在大脑发育过程中，神经元和神经胶质细胞均由脑室区的神经前体细胞分化而来。神经前体细胞的命运受谷氨酸等神经递质的影响。本研究采用钙数字成像法，检测了谷氨酸触发神经前体细胞系细胞内 Ca(2+)信号转导的情况。用谷氨酸处理永生化原代培养神经前体细胞时，其中一部分细胞内 Ca(2+)浓度增加。为了确定谷氨酸触发的细胞内 Ca(2+)信号在神经前体细胞中的作用，我们尝试在无谷氨酸的培养基中培养永生化基底神经节和海马神经前体细胞系。海马（MHP-2）细胞适应了无谷氨酸的培养基，当用谷氨酸处理时，适应的亚系（MHP-2-E1）细胞内 Ca(2+)浓度增加。相比之下，基底神经节神经前体细胞系未能适应无谷氨酸的培养基。这些结果表明，海马和基底神经节神经前体细胞对谷氨酸作为外源性刺激的细胞反应存在差异。
