Division Génie Chimique et Biochimique, Bertin & Cie, Boîte postale 3, 78373, Plaisir Cedex, France.
Cytotechnology. 1989 May;2(2):119-29. doi: 10.1007/BF00386144.
It is generally accepted that the phase of cell decline observed in batch culture of mammalian cells is related to exhaustion of medium nutrients (principally glucose and glutamine) and/or to waste products accumulation. In the present paper, we have studied the influence of glutamine on the proliferation of mouse hybridoma cells. We showed that repeated addition of glutamine prolonged the life span of the culture and significantly increased the secretion of monoclonal antibody. Flow cytometry analysis suggests that this effect of glutamine is related to a delay in cell death rather than to a stimulation of proliferation.Addition of glutamine and glucose failed however to prevent the death of the culture. Determinations of amino acid consumption in glutamine-supplemented samples and experiments carried out with complementary sources of amino acids (e.g. tryptose phosphate) strongly suggest that amino acid supply is a critical factor governing cell growth and productivity.
人们普遍认为，在哺乳动物细胞的分批培养中观察到的细胞衰退阶段与培养基营养物质（主要是葡萄糖和谷氨酰胺）的耗尽和/或废物积累有关。在本文中，我们研究了谷氨酰胺对小鼠杂交瘤细胞增殖的影响。我们表明，重复添加谷氨酰胺可延长培养物的寿命，并显著增加单克隆抗体的分泌。流式细胞术分析表明，谷氨酰胺的这种作用与细胞死亡的延迟有关，而不是增殖的刺激。然而，添加谷氨酰胺和葡萄糖并不能防止培养物的死亡。在添加谷氨酰胺的样品中测定氨基酸消耗和用补充氨基酸来源（例如色氨酸磷酸盐）进行的实验强烈表明，氨基酸供应是决定细胞生长和生产力的关键因素。
