Division of Medical Devices, National Institute of Health Sciences, 1-18-1, Kamiyoga, Setagaya ku, 158-8501, Tokyo, Japan.
Cytotechnology. 2001 Nov;37(3):163-9. doi: 10.1023/A:1020506821201.
Chondrogenic differentiation of mesenchymal cells is generally thought to be initiated by the inductive action of specific growth factors and depends on intimate cell-cell interactions. The aim of our investigation was to characterize the influences of basic fibroblast growth factor (bFGF) and ferroussulfate (FeSO(4)) on proliferation and differentiation of human articular chondrocytes (HAC). This is the first report of the effects of FeSO(4) on chondrogenesis of HAC. Multiplied chondrocytes of hip and shoulder joints were cultured in chondrocyte growth medium supplemented with bFGF, FeSO(4), or both bFGF + FeSO(4) for4weeks. A 20 mul aliquot of a cell suspension containing2 x 10(7) cells ml(-1) was delivered onto each well of 24-well tissue culture plates. Cells cultured with the growth medium only was used as a control. Alamar blue and alcian blue staining were done to determine the chondrocyte proliferation and differentiation, respectively, after 4 weeks. The samples exposed to bFGF, FeSO(4), and combination of both indicated sufficient cell proliferation similar to the control level. Differentiations of the HAC exposed to bFGF, FeSO(4),and bFGF + FeSO(4) were 1.2-, 2.0-, and 2.2-fold of the control, respectively. Therefore, chondrocyte differentiation was significantly enhanced by the addition of FeSO(4) andbFGF + FeSO(4). The combined effects of bFGF and FeSO(4) were additive, rather than synergistic. These results suggest that treatment with ferrous sulfate alone or in combination with basic fibroblast growth factor etc, is a powerful tool to promote the differentiation of HAC for the clinical application.
软骨细胞的软骨分化通常被认为是由特定生长因子的诱导作用启动的，并且依赖于细胞间的密切相互作用。我们研究的目的是研究碱性成纤维细胞生长因子（bFGF）和硫酸亚铁（FeSO4）对人关节软骨细胞（HAC）增殖和分化的影响。这是首次报道 FeSO4 对 HAC 软骨发生的影响。来自髋关节和肩关节的传代软骨细胞在补充有 bFGF、FeSO4 或 bFGF+FeSO4 的软骨细胞生长培养基中培养 4 周。将含有 2x10(7)细胞/ml(-1)的细胞悬液 20mul 加入 24 孔组织培养板的每个孔中。仅用生长培养基培养的细胞用作对照。在第 4 周后，分别通过 Alamar blue 和 alcian blue 染色来确定软骨细胞的增殖和分化。暴露于 bFGF、FeSO4 和两者组合的样本显示出与对照水平相似的足够细胞增殖。暴露于 bFGF、FeSO4 和 bFGF+FeSO4 的 HAC 的分化分别是对照的 1.2、2.0 和 2.2 倍。因此，FeSO4 和 bFGF+FeSO4 的添加显著增强了软骨细胞的分化。bFGF 和 FeSO4 的联合作用是相加的，而不是协同的。这些结果表明，单独使用硫酸亚铁或与碱性成纤维细胞生长因子等联合使用是促进 HAC 分化的有效工具，可用于临床应用。
