Dreyfus J, Brunet-de Carvalho N, Duprez D, Raulais D, Vigny M
Unité INSERM 440, Paris, France.
Exp Cell Res. 1998 May 25;241(1):171-80. doi: 10.1006/excr.1998.4040.
The heparin-binding growth-associated molecule HB-GAM (also named pleiotrophin) and the retinoic acid-induced heparin-binding protein RIHB (chicken midkine) are developmentally regulated proteins forming a new family of heparin-binding molecules with putative functions during cell growth and differentiation. A direct involvement of these molecules during chondrogenesis in vivo was suggested by their patterns of expression. The putative chondrogenic activity of these molecules was investigated in vitro using micromass cultures from chicken limb bud mesenchymal cells. Exogenous HB-GAM, not RIHB, was found to enhance chondrogenesis in this system. These results provide a strong incentive for considering and further investigating the role of this protein in the control of limb cartilage differentiation.
肝素结合生长相关分子HB-GAM（也称为多效生长因子）和视黄酸诱导的肝素结合蛋白RIHB（鸡中期因子）是在发育过程中受调控的蛋白质，它们形成了一个新的肝素结合分子家族，在细胞生长和分化过程中具有假定的功能。这些分子在体内软骨形成过程中的直接参与是由它们的表达模式所提示的。利用鸡胚肢芽间充质细胞的微团培养法在体外研究了这些分子假定的软骨形成活性。结果发现，外源性HB-GAM而非RIHB可增强该系统中的软骨形成。这些结果为考虑并进一步研究该蛋白在肢体软骨分化控制中的作用提供了有力的动机。
