Lee J D, Hwang O, Kim S W, Han S
Department of Biochemistry, University of Ulsan College of Medicine, Songpa-ku, Seoul, Korea.
Life Sci. 1997;61(3):293-9. doi: 10.1016/s0024-3205(97)00385-8.
Responses of rib and ear chondrocytes to basic fibroblast growth factor (bFGF) and transforming growth factor-beta (TGF-beta) were investigated using high density primary culture isolated from the same rabbit. Degrees of tritiated thymidine, leucine, and proline incorporation were used as indicators of DNA, protein and collagen syntheses, respectively. 10 ng/ml bFGF increased thymidine, proline, and leucine incorporation into rib, but not ear, chondrocytes. 1 ng/ml TGF-beta enhanced thymidine incorporation into both chondrocytes but did not affect proline or leucine incorporation into the ear cells. When both growth factors were added simultaneously, both cells showed rises in syntheses of DNA, protein and collagen. Incorporation of [35S]sulfate, used as indicator of proteoglycan synthesis, was elevated by TGF-beta but was reduced by bFGF especially in the rib cells. This inhibitory effect of bFGF was not reversed by cotreatment with TGF-beta in both cell types. Thus, the origin and cellular differentiation states of chondrocytes seem to cause different responses to these growth factors.
利用从同一只兔子分离出的高密度原代培养物，研究了肋骨和耳软骨细胞对碱性成纤维细胞生长因子（bFGF）和转化生长因子-β（TGF-β）的反应。氚标记胸腺嘧啶核苷、亮氨酸和脯氨酸的掺入程度分别用作DNA、蛋白质和胶原蛋白合成的指标。10 ng/ml的bFGF增加了肋骨软骨细胞而非耳软骨细胞中胸腺嘧啶核苷、脯氨酸和亮氨酸的掺入。1 ng/ml的TGF-β增强了两种软骨细胞中胸腺嘧啶核苷掺入，但不影响耳软骨细胞中脯氨酸或亮氨酸的掺入。当同时添加这两种生长因子时，两种细胞的DNA、蛋白质和胶原蛋白合成均增加。用作蛋白聚糖合成指标的[35S]硫酸盐掺入量被TGF-β提高，但被bFGF降低，尤其是在肋骨软骨细胞中。在两种细胞类型中，bFGF的这种抑制作用不会因与TGF-β共同处理而逆转。因此，软骨细胞的来源和细胞分化状态似乎导致对这些生长因子产生不同的反应。
