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1
High-level expression of a foreign gene by a recombinant baculovirus with an expanded host range.利用宿主范围扩大的重组杆状病毒高效表达外源基因。
Cytotechnology. 2000 Feb;32(2):87-92. doi: 10.1023/A:1008166310368.
2
Host range expansion of Autographa californica nuclear polyhedrosis virus (NPV) following recombination of a 0.6-kilobase-pair DNA fragment originating from Bombyx mori NPV.源于家蚕核型多角体病毒的一个0.6千碱基对DNA片段重组后，苜蓿银纹夜蛾核型多角体病毒的宿主范围扩展
J Virol. 1993 Oct;67(10):6234-8. doi: 10.1128/JVI.67.10.6234-6238.1993.
3
The morphology of the polyhedra of a host range-expanded recombinant baculovirus and its parents.宿主范围扩大的重组杆状病毒及其亲本的多面体形态。
Arch Virol. 1998;143(6):1209-14. doi: 10.1007/s007050050368.
4
Expression of a Ligninase by a New Hybrid Baculovirus with Expanded Host Range.一种具有扩大宿主范围的新型杂交杆状病毒对木质素酶的表达
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High-frequency homologous recombination between baculoviruses involves DNA replication.杆状病毒之间的高频同源重组涉及DNA复制。
J Virol. 2003 Dec;77(24):13053-61. doi: 10.1128/jvi.77.24.13053-13061.2003.
6
Expression profiling of baculovirus genes in permissive and nonpermissive cell lines.杆状病毒基因在允许性和非允许性细胞系中的表达谱分析。
Biochem Biophys Res Commun. 2004 Oct 15;323(2):599-614. doi: 10.1016/j.bbrc.2004.08.114.
7
Abortive infection of the baculovirus Autographa californica nuclear polyhedrosis virus in Sf-9 cells after mutation of the putative DNA helicase gene.假定的DNA解旋酶基因突变后，苜蓿银纹夜蛾核型多角体病毒在Sf-9细胞中的流产感染
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Characterization of recombinant Autographa californica nuclear polyhedrosis virus (NPV) expressing the beta-galactosidase gene in both Sf21 and Bm5 cells by Bombyx mori NPV p143 helicase gene.通过家蚕核型多角体病毒p143解旋酶基因在Sf21和Bm5细胞中对表达β-半乳糖苷酶基因的重组苜蓿银纹夜蛾核型多角体病毒（NPV）进行特性鉴定。
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Foreign gene expression by a baculovirus vector with an expanded host range.具有扩大宿主范围的杆状病毒载体对外源基因的表达
J Gen Virol. 1992 Jul;73 ( Pt 7):1877-80. doi: 10.1099/0022-1317-73-7-1877.
10
Construction of a host range-expanded hybrid baculovirus of BmNPV and AcNPV, and knockout of cysteinase gene for more efficient expression.构建家蚕核型多角体病毒（BmNPV）和苜蓿银纹夜蛾核型多角体病毒（AcNPV）宿主范围扩大的杂交杆状病毒，并敲除半胱氨酸酶基因以实现更高效表达。
Sci China C Life Sci. 2004 Oct;47(5):406-15. doi: 10.1360/03yc0128.

本文引用的文献

1
Optimizing the baculovirus expression vector system.优化杆状病毒表达载体系统。
Methods. 2011 Sep;55(1):52-7. doi: 10.1016/j.ymeth.2011.06.011. Epub 2011 Sep 13.
2
Nucleotide sequence of the polyhedrin gene of Autographa californica nuclear polyhedrosis virus.苜蓿银纹夜蛾核型多角体病毒多角体蛋白基因的核苷酸序列
Virology. 1983 Dec;131(2):561-5. doi: 10.1016/0042-6822(83)90522-6.
3
Effect of silkworm hemolymph on the expression of E. coli beta-galactosidase in insect cell lines infected with recombinant baculoviruses.家蚕血淋巴对感染重组杆状病毒的昆虫细胞系中大肠杆菌β-半乳糖苷酶表达的影响。
Mol Cells. 1997 Aug 31;7(4):572-4.
4
Host range expansion of Autographa californica nuclear polyhedrosis virus (NPV) following recombination of a 0.6-kilobase-pair DNA fragment originating from Bombyx mori NPV.源于家蚕核型多角体病毒的一个0.6千碱基对DNA片段重组后，苜蓿银纹夜蛾核型多角体病毒的宿主范围扩展
J Virol. 1993 Oct;67(10):6234-8. doi: 10.1128/JVI.67.10.6234-6238.1993.
5
Extension of Autographa californica nuclear polyhedrosis virus host range by interspecific replacement of a short DNA sequence in the p143 helicase gene.通过种间替换苜蓿银纹夜蛾核型多角体病毒p143解旋酶基因中的一段短DNA序列来扩展其宿主范围
Proc Natl Acad Sci U S A. 1994 Jan 4;91(1):48-52. doi: 10.1073/pnas.91.1.48.
6
Identification of genes involved in DNA replication of the Autographa californica baculovirus.鉴定参与苜蓿银纹夜蛾核型多角体病毒DNA复制的基因。
Proc Natl Acad Sci U S A. 1994 Nov 8;91(23):11212-6. doi: 10.1073/pnas.91.23.11212.
7
Polyhedrin gene of Bombyx mori nuclear polyhedrosis virus.家蚕核型多角体病毒的多角体蛋白基因
J Virol. 1985 May;54(2):436-45. doi: 10.1128/JVI.54.2.436-445.1985.
8
Expression of foreign genes in insects using baculovirus vectors.使用杆状病毒载体在昆虫中表达外源基因。
Annu Rev Entomol. 1989;34:351-72. doi: 10.1146/annurev.en.34.010189.002031.
9
High level expression of nonfused foreign genes with Autographa californica nuclear polyhedrosis virus expression vectors.利用苜蓿银纹夜蛾核型多角体病毒表达载体实现非融合外源基因的高水平表达。
Virology. 1989 May;170(1):31-9. doi: 10.1016/0042-6822(89)90348-6.
10
Host range expansion by recombination of the baculoviruses Bombyx mori nuclear polyhedrosis virus and Autographa californica nuclear polyhedrosis virus.通过家蚕核型多角体病毒和苜蓿银纹夜蛾核型多角体病毒重组实现杆状病毒宿主范围的扩展。
J Virol. 1991 Jul;65(7):3625-32. doi: 10.1128/JVI.65.7.3625-3632.1991.

利用宿主范围扩大的重组杆状病毒高效表达外源基因。

High-level expression of a foreign gene by a recombinant baculovirus with an expanded host range.

机构信息

Division of Applied Biology and Chemistry, College of Agriculture and Life Sciences, Seoul National University, Suwon, 441-744, Korea.

出版信息

Cytotechnology. 2000 Feb;32(2):87-92. doi: 10.1023/A:1008166310368.

DOI:10.1023/A:1008166310368
PMID:19002970
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3449685/
Abstract

The usefulness of host range expanded viruses as an expressionvector system was investigated by following the expression ofthe E. coli lacZ gene. The host range expanded recombinantviruses were obtained from Sf-21 or BmN-4 cells coinfected withAutographa californica and Bombyx mori nuclearpolyhedrosis viruses. Among the host range expanded viruses,RecB-8 and RecS-B6 have similar enzyme digestion profiles butdifferent infection characteristics in cells. Therefore, tostudy the foreign gene expression efficiency of these twoviruses, we constructed recombinant viruses RecB8-LacZ andRecSB6-LacZ containing the lacZ gene instead of the polyhedringene. Also, the host range expanded recombinant AcNPV, Bac-BH,containing lacZ gene in the polyhedrin gene locus was constructedby substitution of the 0.6 kb region within the helicase gene ofBacPAK6 with that of BmNPV. beta-Galactosidase expressionefficiency by these viruses were determined and compared in Sf-21and BmN-4 cells. The result showed that Bac-BH has highexpression efficiency only in Sf-21 cells, whereas RecB8-LacZhas high expression efficiency both in Sf-21 and BmN-4 cells.Also, in BmN-4 cells, beta-galactosidase expressionefficiency of RecB8-LacZ was higher than that of recombinantBmNPV (BmK1-LacZ containing lacZ gene in polyhedrin gene locus).In addition, the expression efficiency was not correlated withvirus titer.

摘要

研究了宿主范围扩展病毒作为表达载体系统的有用性，方法是跟踪大肠杆菌 lacZ 基因的表达。从 Sf-21 或 BmN-4 细胞中共同感染美洲棉铃象鼻虫和家蚕核多角体病毒获得了宿主范围扩展的重组病毒。在宿主范围扩展的病毒中，RecB-8 和 RecS-B6 具有相似的酶切图谱，但在细胞中的感染特性不同。因此，为了研究这两种病毒的外源基因表达效率，我们构建了含有 lacZ 基因而不是多角体基因的重组病毒 RecB8-LacZ 和 RecSB6-LacZ。此外，通过用 BmNPV 的螺旋酶基因内的 0.6 kb 区域取代 BacPAK6 中的螺旋酶基因，构建了含有 lacZ 基因的宿主范围扩展的重组 AcNPV Bac-BH。在 Sf-21 和 BmN-4 细胞中测定了这些病毒的β-半乳糖苷酶表达效率并进行了比较。结果表明，Bac-BH 仅在 Sf-21 细胞中具有高表达效率，而 RecB8-LacZ 在 Sf-21 和 BmN-4 细胞中均具有高表达效率。此外，在 BmN-4 细胞中，RecB8-LacZ 的β-半乳糖苷酶表达效率高于含有多角体基因座 lacZ 基因的重组 BmNPV（BmK1-LacZ）。此外，表达效率与病毒滴度无关。