Woo S D, Kim W J, Kim H S, Choi J Y, Jin B R, Kang S K
Department of Agricultural Biology, College of Agriculture and Life Sciences, Seoul National University, Suwon, Korea.
Mol Cells. 1997 Aug 31;7(4):572-4.
The effects of silkworm hemolymph on the expression of foreign genes by recombinant baculoviruses in cell lines were studied. The expression efficiency of beta-galactosidase by recombinant virus containing the E. coli lacZ gene at various concentrations of hemolymph and FBS was determined in BmN and Sf cell lines. The addition of hemolymph to the medium containing FBS accelerated the expression of beta-galactosidase by recombinant viruses in both cells. It was more effective in BmN cells than in Sf cells. Hemolymph was most effective in enhancing virus multiplicity under conditions of 5% FBS.
研究了家蚕血淋巴对重组杆状病毒在细胞系中表达外源基因的影响。在BmN和Sf细胞系中，测定了含有大肠杆菌lacZ基因的重组病毒在不同浓度血淋巴和胎牛血清（FBS）条件下β-半乳糖苷酶的表达效率。在含有FBS的培养基中添加血淋巴可加速重组病毒在两种细胞中β-半乳糖苷酶的表达。在BmN细胞中比在Sf细胞中更有效。在5%FBS条件下，血淋巴在提高病毒增殖率方面最有效。
