Woo S D, Kim W J, Kim H S, Jin B R, Lee Y H, Kang S K
Department of Agricultural Biology, College of Agriculture & Life Sciences, Seoul National University, Suwon, Korea.
Arch Virol. 1998;143(6):1209-14. doi: 10.1007/s007050050368.
The host range-expanded recombinant baculovirus, RecB-8 was isolated from BmN-4 cells coinfected with Autographa californica and Bombyx mori nuclear polyhedrosis viruses. Its genome was compared with those of its parents by restriction endonuclease digestion and their polyhedra compared in an electron microscope. Interestingly, the polyhedra of RecB-8 were tetrahedral although the polyhedrin gene was the same as that of the BmNPV parent which has icosahedral polyhedra. Thus the morphology of the RecB-8 polyhedra resulted from host cell factors and/or another viral genome in the host cells.
宿主范围扩大的重组杆状病毒RecB-8是从同时感染苜蓿银纹夜蛾核型多角体病毒和家蚕核型多角体病毒的BmN-4细胞中分离得到的。通过限制性内切酶消化将其基因组与其亲本的基因组进行比较，并在电子显微镜下比较它们的多角体。有趣的是，尽管RecB-8的多角体蛋白基因与具有二十面体多角体的家蚕核型多角体病毒亲本的相同，但其多角体却是四面体的。因此，RecB-8多角体的形态是由宿主细胞因子和/或宿主细胞中的另一种病毒基因组导致的。
