Department of Agrobioscience, Graduate School of Agricultural Science, Kobe University, 1-1 Rokkodai, Nada, Kobe, 657 8501, Japan.
Cytotechnology. 2007 Dec;55(2-3):103-8. doi: 10.1007/s10616-007-9107-y. Epub 2007 Oct 31.
Some of inositol derivatives have been reported to help the action of insulin stimulating glucose uptake in skeletal muscle cells. Rat L6 myotubes were employed in an attempt to develop an in vitro model system for investigation of the possible insulin-like effect of eight inositol derivatives, namely allo-inositol, D: -chiro-inositol L: -chiro-inositol, epi-inositol, muco-inositol, myo-inositol, scyllo-inositol and D: -pinitol. At a higher concentration of 1 mM seven inositol derivatives other than myo-inositol were able to stimulate glucose uptake, while at 0.1 mM only D: -chiro-inositol, L: -chiro-inositol, epi-inositol and muco-inositol could induce glucose uptake, indicating their significant insulin-mimetic activity. Immunoblot analyses revealed that at least D: -chiro-inositol, L: -chiro-inositol, epi-inositol, muco-inositol and D: -pinitol were able to induce translocation of glucose transporter 4 (GLUT4) to plasma membrane not only in L6 myotubes but also in skeletal muscles of rats ex vivo. These results demonstrated that L6 myotubes appeared efficient as an in vitro system to identify inositol derivatives exerting an insulin-like effect on muscle cells depending on the induced translocation of GLUT4.
一些肌醇衍生物已被报道有助于胰岛素刺激骨骼肌细胞葡萄糖摄取的作用。我们采用大鼠 L6 肌管，试图建立一个体外模型系统，以研究八种肌醇衍生物（别肌醇、D：-表肌醇、L：-表肌醇、内肌醇、粘肌醇、肌醇、鲨肌醇和 D：-松醇）可能具有的胰岛素样作用。在 1mM 的较高浓度下，除肌醇以外的七种肌醇衍生物能够刺激葡萄糖摄取，而在 0.1mM 时，只有 D：-表肌醇、L：-表肌醇、内肌醇和粘肌醇能够诱导葡萄糖摄取，表明它们具有显著的胰岛素模拟活性。免疫印迹分析显示，至少 D：-表肌醇、L：-表肌醇、内肌醇、粘肌醇和 D：-松醇不仅能够诱导 L6 肌管，还能够诱导大鼠骨骼肌中葡萄糖转运蛋白 4（GLUT4）向质膜转位。这些结果表明，L6 肌管作为一种体外系统，能够有效地识别出对肌肉细胞具有胰岛素样作用的肌醇衍生物，这取决于 GLUT4 的诱导转位。
