Identification of Phytochemicals in Bioactive Extracts of Growing in Australia.

growing in Australia has not been fully investigated for its bioactive phytochemicals. Sequential polarity-based extraction was employed to provide four different extracts from individual parts of . Bioactive extracts were determined using in vitro antioxidant and yeast α-glucosidase inhibitory assays. Methanolic extracts from barks, leaves, and flowers are the most active and have no toxicity against 3T3-L1 adipocytes. Compound isolation of bioactive extracts provided us with ten compounds. Among them are two novel natural products; naringenin-7-O-α--arabinopyranoside and ()-3-hydroxy-5-(2-aminoethyl) dihydrofuran-2(3)-one . -(+)-pinitol (from barks and flowers), (-)-pinitol (exclusively from leaf), and 2,4-di--butylphenol are known natural products and new to . (-)-Epicatechin , quercitrin , and myricitrin showed potent antioxidant activities consistently in DPPH and ABTS assays. (-)-Epicatechin (IC = 63.58 μM)-(+)-pinitol (IC = 74.69 μM), and naringenin (IC = 89.71 μM) are the strong inhibitors against the α-glucosidase enzyme. The presence of these compounds supports the activities exerted in our methanolic extracts. The presence of 2,4-di--butylphenol may support the reported allelopathic and antifungal activities. The outcome of this study indicates the potential of Australian as a rich source of bioactive compounds for drug discovery targeting type 2 diabetes.