通过添加甘油增强 CHO 细胞中重组 M-CSF 的生产：模型和验证。
Enhanced recombinant M-CSF production in CHO cells by glycerol addition: model and validation.
机构信息
Graduate Institute of Biochemical and Biomedical Engineering, Chang Gung University, 259, Wen-Hwa first Road, Kwei-Shan, Taoyuan, 333, Taiwan,
出版信息
Cytotechnology. 2007 Jun;54(2):89-96. doi: 10.1007/s10616-007-9078-z. Epub 2007 Jun 13.
Addition of stimulatory chemical such as glycerol was found to increase recombinant protein production in Chinese hamster ovary (CHO) cells. However, glycerol influenced cell mitosis and reduced cell growth rate. We developed a controlled proliferation strategy to utilize the stimulation of glycerol on recombinant protein production and mitigate the problem of growth inhibition. The approach is to apply a two-stage process, where cells are cultured without glycerol for a period of time in order to obtain enough cell density and then glycerol is added to achieve high specific productivity. In addition, a model for predicting the profiles of cell proliferation and recombinant protein production was developed and validated. A two-stage process, addition of 1% glycerol after 1 day of growth, could increase the final production of macrophage-colony stimulating factor (M-CSF) by 38% compared with the value obtained without addition of glycerol.
添加刺激化学物质，如甘油，被发现可以增加中国仓鼠卵巢（CHO）细胞中重组蛋白的产量。然而，甘油会影响细胞有丝分裂，降低细胞生长速度。我们开发了一种控制增殖的策略，以利用甘油对重组蛋白生产的刺激作用，并减轻生长抑制的问题。该方法采用两阶段工艺，先在无甘油的条件下培养一段时间，以获得足够的细胞密度，然后再添加甘油以实现高比生产率。此外，还开发并验证了预测细胞增殖和重组蛋白生产曲线的模型。与不添加甘油相比，在培养 1 天后添加 1%甘油的两阶段工艺可使巨噬细胞集落刺激因子（M-CSF）的最终产量提高 38%。
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