Faculty of Agriculture, Ehime University, Matsuyama, Ehime, 790-8566, Japan.
Cytotechnology. 2005 Jan;47(1-3):51-7. doi: 10.1007/s10616-005-3764-5.
The cationic vesicle composed of Span80 and cationic peptide lipid (CPL) was prepared. The cytotoxicity of the Span80-CPL cationic vesicle was very low compared with Span80 vesicle. Antibody was able to be immobilized on vesicle surface by mediation of protein A. The antigen targeting ability of the antibody-immobilized vesicle (immunovesicle) derived from antibody was evaluated. Our results suggested that the Span80-CPL immunovesicles specifically associate with target cells by the antibody mediation, and the substance capsulated in immunovesicle was transferred into the target cells. This means that the Span80-CPL immunovesicle is expected to achieve a high local concentration of an encapsulated drug at the target.
制备了由 Span80 和阳离子肽脂质（CPL）组成的阳离子囊泡。与 Span80 囊泡相比，Span80-CPL 阳离子囊泡的细胞毒性非常低。抗体可以通过蛋白 A 的介导固定在囊泡表面。评估了源自抗体的抗体固定囊泡（免疫囊泡）的抗原靶向能力。我们的结果表明，Span80-CPL 免疫囊泡通过抗体介导特异性与靶细胞结合，并且免疫囊泡中包封的物质被转移到靶细胞中。这意味着 Span80-CPL 免疫囊泡有望在靶部位实现包封药物的高局部浓度。
