Zuo Li, Zhang Fengchun, Xu Yingchun
Department of Oncology, Fudan University Shanghai Cancer Center, Minhang Branch Shanghai, China.
Department of Oncology, Suzhou Kowloon Hospital, Shanghai Jiao Tong University Suzhou, China.
Int J Clin Exp Pathol. 2019 Jan 1;12(1):205-211. eCollection 2019.
Cationic polymeric liposomes (CPLs) were successfully prepared using a tumor-targeting EGF protein modification for the systemic delivery of the p53 gene in ovarian cancer. These functional CPL nanoparticles (NPs) can be used as effective gene delivery vectors and are composed of an EGF derivative (EGF-GHDC), cholesterol, and DOPE, which have lower gene transfection efficiencies compared with Lipofectamine 2000. The increased therapeutic efficiency of p53 gene-loaded EGF-CPL was tested in SKOV3 cells and compared with p53 gene-loaded CPL and free p53 gene in solution. In addition, EGF-CPL (effective diameter: 95.01±4.05 nm; polydispersity index: 0.270) significantly enhanced luciferase gene expression in vivo 3 days post-injection of pGL-3/NPs complexes. In conclusion, EGF-targeted CPL has the potential for use as an effective drug delivery system.
通过对表皮生长因子（EGF）蛋白进行肿瘤靶向修饰，成功制备了阳离子聚合物脂质体（CPL），用于卵巢癌中p53基因的全身递送。这些功能性CPL纳米颗粒（NP）可作为有效的基因递送载体，由一种EGF衍生物（EGF-GHDC）、胆固醇和二油酰磷脂酰乙醇胺（DOPE）组成，与Lipofectamine 2000相比，其基因转染效率较低。在SKOV3细胞中测试了负载p53基因的EGF-CPL的治疗效果增强情况，并与负载p53基因的CPL和溶液中的游离p53基因进行了比较。此外，在注射pGL-3/NP复合物3天后，EGF-CPL（有效直径：95.01±4.05 nm；多分散指数：0.270）在体内显著增强了荧光素酶基因表达。总之，EGF靶向CPL有潜力用作有效的药物递送系统。
