Division of Chemical Engineering, Graduate School of Engineering Science, Osaka University, 1-3 Machikaneyama-cho, 560-8531, Toyonaka, Osaka, Japan.
Cytotechnology. 2005 Jan;47(1-3):127-31. doi: 10.1007/s10616-005-3749-4.
In the successive cultures of human keratinocyte cells, cellular motions of extension and rotation were analyzed based on observation of the individual cells, to evaluate the proliferative potential in a whole cell population. In lag phases of the serial cultures, an extension index of individual cells, R(E), was defined as an average spreading rate divided by initial cell area for each cell. The mean value of R(E) was found to relate to prolongation of lag time; namely it decreased with increasing passage number in the successive cultures approaching cellular senescence. During the courses of the cultures, the rotation rate of paired cells was also measured through time-lapse observation. The mean value of rotation rate, [Formula: see text], decreased with an increase in doubling time caused by the progress of cellular age, reaching an almost constant value of [Formula: see text] h(-1) in the cultures with prolonged doubling time of over 59 h. It was concluded that the indices determined from the motions of individual cells, R(E) and [Formula: see text], were correlated with the lag time and doubling time, respectively, which are growth parameters varied with the vitality of the cells approaching cellular senescence.
在连续培养的人类角质形成细胞中，通过观察单个细胞来分析细胞的延伸和旋转运动，以评估整个细胞群体的增殖潜力。在连续培养的潜伏期，定义单个细胞的延伸指数 R(E)为每个细胞的平均扩展速率除以初始细胞面积。发现 R(E)的平均值与潜伏期的延长有关；即随着传代次数的增加，R(E)的值逐渐减小，接近细胞衰老。在培养过程中，还通过延时观察测量了配对细胞的旋转速率。旋转速率的平均值 [Formula: see text]随着细胞年龄的增长导致倍增时间的增加而降低，在倍增时间超过 59 h 的培养物中，达到几乎恒定的值 [Formula: see text] h(-1)。结论是，从单个细胞运动中确定的指数 R(E)和 [Formula: see text]与潜伏期和倍增时间分别相关，这些参数是随细胞活力变化而变化的，接近细胞衰老。
