高通量终点法检测活体细胞计数。
A High-throughput End-point Assay for Viable Mammalian Cell Estimation.
机构信息
Graduate School of the Chinese Academy of Sciences, Shanghai, China.
出版信息
Cytotechnology. 2005 Sep;49(1):51-8. doi: 10.1007/s10616-005-5545-6.
Abstract
A single wavelength colorimetric microplate-based assay was developed using non-cytotoxic dye resazurin for the estimation of viable cell concentrations of Chinese hamster ovary (CHO) and hybridoma cells. Experimental results showed variations in pH and temperature caused by cell cultivation and assay operations were well tolerated. Cell concentrations can be effectively determined in the range of 10(5)-10(7) cells ml(-1) using a microplate reader at the wavelength of 605 nm. This assay can be performed in a high-throughput manner such that a large number of cell culture samples can be screened within a relatively short time frame. When used together with a cell culture system of high-throughput format, it may have potential utilities in applications such as cell culture medium formulation and optimization.
摘要
开发了一种基于单波长比色微板的 assay，使用非细胞毒性染料 resazurin 来估计中国仓鼠卵巢 (CHO) 和杂交瘤细胞的活细胞浓度。实验结果表明，细胞培养和 assay 操作引起的 pH 和温度变化能够很好地耐受。使用 605nm 波长的微孔板读数仪，细胞浓度可在 10(5)-10(7)细胞/ml(-1)的有效范围内进行测定。这种 assay 可以以高通量的方式进行，使得可以在相对较短的时间内筛选大量的细胞培养样品。当与高通量格式的细胞培养系统一起使用时，它可能在细胞培养基配方和优化等应用中具有潜在的用途。
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