用于动物细胞培养中培养基优化的带有集成氧传感功能的微孔板。
Microplates with integrated oxygen sensing for medium optimization in animal cell culture.
机构信息
Biochemical Engineering Institute, University of Saarland, Saarbruecken, Germany.
出版信息
Cytotechnology. 2004 Sep;46(1):1-8. doi: 10.1007/s10616-004-6401-9. Epub 2005 Jun 16.
Abstract
A new approach using microtiter plate cultivation with on-line measurement of dissolved oxygen (DO) was applied for medium optimization of mammalian cell culture. Applying dynamic liquid phase balance, oxygen uptake rates were calculated from the DO level and used as an indicator for culture viability. The developed method was successfully applied to optimization of the concentration of glucose, glutamine and inorganic salts for cultivation of a Chinese Hamster Ovary (CHO) cell line. Using 2(3) full factorial central composite design, the optimum medium composition could be identified in one single run. The concentration of inorganic salts had a significant influence on cultivation. The developed method exhibits high potential to improve procedures of medium optimization for animal cell cultivation by allowing the investigation of large sets of potentially important variables in short time and with reduced effort.
摘要
一种使用微量滴定板培养并在线测量溶解氧 (DO) 的新方法被应用于哺乳动物细胞培养的培养基优化。应用动态液相平衡，通过 DO 水平计算氧摄取率，并用作培养活力的指标。所开发的方法成功应用于优化葡萄糖、谷氨酰胺和无机盐的浓度，用于培养中国仓鼠卵巢 (CHO) 细胞系。使用 2(3) 完全因子中心组合设计，可以在一次运行中确定最佳培养基组成。无机盐的浓度对培养有显著影响。该方法通过允许在短时间内以较小的工作量研究大量潜在重要变量，具有很大的潜力来改进动物细胞培养的培养基优化程序。
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