Aber Instruments Ltd., Science Park, Aberyswyth, UK,
Cytotechnology. 2006 Mar;50(1-3):35-48. doi: 10.1007/s10616-005-3974-x. Epub 2006 Jun 23.
In this work, radio-frequency (RF) impedance is reviewed as a method for monitoring and controlling cell culture manufacturing processes. It is clear from the many publications cited that RF Impedance is regarded as an accurate and reliable method for measuring the live cell bio-volume both on-line and off-line and the technology is also sutable for animal cells in suspension, attached to micro-carriers or immobilized in fixed beds. In cGMP production, RF Impedance is being used in three main areas. Firstly, it is being used as a control instrument for maintaining consistent perfusion culture allowing the bioreactor to operate under optimum conditions for maximum production of recombinant proteins. In the second application it has not replaced traditional off-line live cell counting techniques but it is being used as an additional monitoring tool to check product conformance. Finally, RF Impedance is being used to monitor the concentration of live cells immobilized on micro-carriers or packed beds in cGMP processes where traditional off-line live cell counting methods are inaccurate or impossible to perform.
在这项工作中，射频（RF）阻抗被视为监测和控制细胞培养制造过程的一种方法。从引用的许多出版物中可以清楚地看出，RF 阻抗被认为是一种准确可靠的方法，可用于在线和离线测量活细胞的生物体积，并且该技术也适用于悬浮的动物细胞、附着在微载体上或固定在固定床上的动物细胞。在 cGMP 生产中，RF 阻抗主要用于三个领域。首先，它被用作维持一致的灌注培养的控制仪器，使生物反应器在最佳条件下运行，以最大限度地生产重组蛋白。在第二种应用中，它并没有取代传统的离线活细胞计数技术，但它被用作额外的监测工具，以检查产品是否符合要求。最后，RF 阻抗被用于监测固定在微载体或填充床中的活细胞的浓度，在 cGMP 工艺中，传统的离线活细胞计数方法不准确或不可能进行。
