Spier R E, Whiteside J P
Biotechnol Bioeng. 1976 May;18(5):659-67. doi: 10.1002/bit.260180506.
Methods are described which make possible the production of foot-and-mouth disease (FMD) virus from BHK 21 C13 monolayer cells which have been grown on the surface of serum coated DEAE Sephadex A50 beads. The yield of cells and their susceptibility to infection by FMD virus are equivalent to conventional Roux monolayer systems. The potential for the commercial application of the DEAE Sephadex A50 system is discussed in relation to other unit process monolayer systems and in particular to the system in which cells are cultured in a deep bed of small glass spheres.
本文描述了一些方法，这些方法能够从生长在血清包被的DEAE葡聚糖A50珠表面的BHK 21 C13单层细胞中生产口蹄疫（FMD）病毒。细胞产量及其对口蹄疫病毒感染的敏感性与传统的鲁氏单层系统相当。文中还讨论了DEAE葡聚糖A50系统相对于其他单元过程单层系统，特别是相对于在小玻璃珠深床中培养细胞的系统的商业应用潜力。
