Animal Cell Biotechnology (, Université Libre de Bruxelles, C.P. 160/17, 1050, Brussels, Belgium.
Cytotechnology. 2001 Jul;36(1-3):49-53. doi: 10.1023/A:1014041003617.
By adsorbing poly(N-isopropylacrylamide) (PNIPAAm) from an aqueous solution onto oxidised polystyrene without the need for grafting the polymer to the surface, we showed here that cells(CHO-K1) adhere and grow well at 37 degrees C and are detached by lowering the temperature to 10 degrees C without any other deleterious treatment. Both bacterial culture grade polystyrene Petri dishes and polystyrene beads (120 to 250mum diameters) commercially available used in static conditions of growth were tested with similar results. The contact angle of modified Petri dishes with a water droplet increases from 36 to 58 degrees when the temperature is raised from 25 to 37 degrees C indicating change in hydrophilicity of the surface as a function of temperature.
通过从水溶液中吸附聚（N-异丙基丙烯酰胺）（PNIPAAm）到氧化聚苯乙烯上，而无需将聚合物接枝到表面，我们在这里表明，细胞（CHO-K1）在 37°C 下粘附和生长良好，并且可以通过将温度降低到 10°C 而无需任何其他有害处理来分离。在静态生长条件下使用的商业上可获得的细菌培养级聚苯乙烯培养皿和聚苯乙烯珠（直径 120 至 250μm）均进行了测试，结果相似。当温度从 25°C升高到 37°C时，带有水滴的改性培养皿的接触角从 36 度增加到 58 度，表明表面亲水性随温度而变化。
