从一名成人 T 细胞白血病患者外周血淋巴细胞来源的 B 细胞系中建立产生针对癌症的人源特异性抗体的杂交瘤。
Establishment of hybridomas producing cancer specific human antibodies from B cell line derived from PBL of a patient with adult T cell leukemia.
机构信息
Department of Genetic Resources Technology, Kyushu University, Fukuoka, 812-8581, Japan.
出版信息
Cytotechnology. 2001 Jul;36(1-3):171-7. doi: 10.1023/A:1014049205434.
Adult T cell leukemia (ATL) is a malignant disease characterized by tumorous proliferation of CD4(+) T cells infected with retrovirus human T cell leukemia virus Type-I (HTLV-I) and concurs with an autoimmune disease and cancer due to attenuated immune response. In this study, we established ATL patient derived B-cell line TM-1 producing cancer-specific IgM antibodies, and further characterized its antigen specificity by establishing hybridomas fused with human-mouse origin hetero-myeloma cell line RF-S1. We established three hybridoma cell lines termed 2E12, 3E9, and 3E10, which continuously secreted human IgM antibodies. Immunohistochemical staining of formalin-fixed tissue section using antibodies secreted from these hybridomas showed that these antibodies specifically recognized tumor sites of human colon adenocarcinomas. Antibody produced from hybridoma 3E9 bound to some of leukemic cell lines, but not to normal human PBL, which was evidenced by the flow cytometric analysis, indicating that antibody produced from 3E9 recognizes cell surface antigen specifically expressed in the leukemic cells.
成人 T 细胞白血病 (ATL) 是一种恶性疾病，其特征是感染逆转录病毒人类 T 细胞白血病病毒 I 型 (HTLV-I) 的 CD4(+) T 细胞肿瘤性增殖，并伴有自身免疫性疾病和癌症，原因是免疫反应减弱。在这项研究中，我们建立了产生癌症特异性 IgM 抗体的 ATL 患者来源的 B 细胞系 TM-1，并通过与人鼠来源的异源骨髓瘤细胞系 RF-S1 融合建立杂交瘤进一步表征其抗原特异性。我们建立了三个杂交瘤细胞系，分别称为 2E12、3E9 和 3E10，它们持续分泌人 IgM 抗体。使用这些杂交瘤分泌的抗体对福尔马林固定组织切片进行免疫组织化学染色显示，这些抗体特异性识别人结肠腺癌的肿瘤部位。杂交瘤 3E9 产生的抗体与一些白血病细胞系结合，但不与正常人外周血淋巴细胞 (PBL) 结合，这一点通过流式细胞分析得到了证实，表明 3E9 产生的抗体识别白血病细胞中特异性表达的细胞表面抗原。
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