采用磁共振波谱和成像技术分析固定床生物反应器中 CHO-K1 细胞的生长。
Analysis of CHO-K1 cell growth in a fixed bed bioreactor using magnetic resonance spectroscopy and imaging.
机构信息
Department of Biochemistry, University of Cambridge, Old Addenbrookes Site, 80 Tennis Court Road, Cambridge, CB2 1GA, UK.
出版信息
Cytotechnology. 1999 Jul;30(1-3):121-32. doi: 10.1023/A:1008039011960.
Non-invasive magnetic resonance imaging and spectroscopy techniques have been used to monitor the growth and distribution of Chinese hamster ovary K1 cells growing in a fixed bed bioreactor composed of macroporous carriers. Diffusion-weighted 1H magnetic resonance spectroscopy was used to monitor the volume fraction of the bioreactor occupied by the cells and diffusion-weighted 1H magnetic resonance imaging was used to map cell distribution. The imaging measurements demonstrated that cell growth in the bioreactor was heterogeneous, with the highest cell densities being found at the surface of the carriers. The increase in the volume fraction occupied by the cells during cell growth showed a close correlation with bioreactor ATP content measured using 31P magnetic resonance spectroscopy. These magnetic resonance measurements, in conjunction with measurements of bioreactor glucose consumption, allowed estimation of the specific glucose consumption rate. This declined during the culture, in parallel with medium glucose concentration.
非侵入性磁共振成像和光谱技术已被用于监测在由大孔载体组成的固定床生物反应器中生长的中国仓鼠卵巢 K1 细胞的生长和分布。扩散加权 1H 磁共振波谱用于监测生物反应器中细胞占据的体积分数，扩散加权 1H 磁共振成像用于绘制细胞分布。成像测量表明，生物反应器中的细胞生长不均匀，载体表面的细胞密度最高。细胞生长过程中细胞占据的体积分数的增加与使用 31P 磁共振光谱法测量的生物反应器 ATP 含量密切相关。这些磁共振测量结果与生物反应器葡萄糖消耗测量结果相结合，可估算出特定的葡萄糖消耗率。在培养过程中，该速率与培养基葡萄糖浓度呈平行下降趋势。
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