Schering-Plough Research Institute, 1011 Morris Avenue, Union, NJ, 07083, USA.
Cytotechnology. 1999 Jul;30(1-3):149-58. doi: 10.1023/A:1008025016272.
This work describes a novel bioreactor system for the cultivation of animal, insect, and plant cells using wave agitation induced by a rocking motion. This agitation system provides good nutrient distribution, off-bottom suspension, and excellent oxygen transfer without damaging fluid shear or gas bubbles. Unlike other cell culture systems, such as spinners, hollow-fiber bioreactors, and roller bottles, scale-up is simple, and has been demonstrated up to 100 L of culture volume. The bioreactor is disposable, and therefore requires no cleaning or sterilization. Additions and sampling are possible without the need for a laminar flow cabinet. The unit can be placed in an incubator requiring minimal instrumentation. These features dramatically lower the purchase cost, and operating expenses of this laboratory/pilot scale cell cultivation system. Results are presented for various model systems: 1) recombinant NS0 cells in suspension; 2) adenovirus production using human 293 cells in suspension; 3) Sf9 insect cell/baculovirus system; and 4) human 293 cells on microcarrier. These examples show the general suitability of the system for cells in suspension, anchorage-dependent culture, and virus production in research and GMP applications.
这项工作描述了一种新型生物反应器系统，用于使用摇床运动引起的波浪搅拌来培养动物、昆虫和植物细胞。这种搅拌系统提供了良好的营养分布、离底悬浮和优秀的氧气传递，而不会损坏流体剪切或气泡。与其他细胞培养系统（如旋转器、中空纤维生物反应器和滚瓶）不同，放大简单，已证明可达到 100 L 的培养体积。生物反应器是一次性的，因此不需要清洁或消毒。添加和取样是可能的，而不需要层流柜。该单元可以放置在需要最少仪器的培养箱中。这些特点极大地降低了该实验室/中试规模细胞培养系统的购买成本和运营成本。为各种模型系统提供了结果：1）悬浮培养的重组 NS0 细胞；2）悬浮培养的人 293 细胞生产腺病毒；3）Sf9 昆虫细胞/杆状病毒系统；4）微载体上的人 293 细胞。这些例子表明，该系统普遍适用于悬浮培养的细胞、锚定依赖性培养和病毒生产的研究和 GMP 应用。
