Singh V
Biotechnology Development, Schering-Plough Research Institute, 1011 Morris Avenue, Union, New Jersey 07083.
Biotechnol Bioeng. 1996 Nov 5;52(3):443-8. doi: 10.1002/(SICI)1097-0290(19961105)52:3<443::AID-BIT12>3.0.CO;2-K.
Measurement of oxygen uptake rate is useful in assessing growth, viability, and metabolic activity. In cell culture, however, the oxygen demand is extremely small (typically 0.1-0.3 mM O(2)L-h) and is very difficult to measure accurately using conventional offgas analysis. In many industrial submerged cell culture systems, dissolved oxygen levels are controlled between preset limits by intermittent sparging of air or oxygen. This article describes a computational method for the automatic online determination of oxygen uptake from the dynamic dissolved oxygen probe response. Experimental measurements show that for a typical hybridoma culture, specific oxygen demand is 0.15 mM O(2)/10(9) cells/h. (c) 1996 John Wiley & Sons, Inc.
氧摄取率的测量对于评估细胞生长、活力和代谢活性很有用。然而，在细胞培养中，氧需求量极小（通常为0.1 - 0.3 mM O₂/L·h），使用传统的尾气分析很难准确测量。在许多工业深层细胞培养系统中，通过间歇性鼓入空气或氧气将溶解氧水平控制在预设限度之间。本文描述了一种根据动态溶解氧探头响应自动在线测定氧摄取的计算方法。实验测量表明，对于典型的杂交瘤培养，比氧需求量为0.15 mM O₂/10⁹细胞/h。(c) 1996约翰·威利父子公司。
