Habib F A
Department of Obstetric and Gynecology, Faculty of Medicine, Taibah University, Saudi Arabia.
J Obstet Gynaecol. 2008 Aug;28(6):593-5. doi: 10.1080/01443610802344332.
Serological immune profile in cases of Toxoplasma infection is heterogeneous, and responses may be delayed or fail to be represented: this makes it an unreliable method for diagnosis and/or treatment follow-up. Therefore, the present study relied on a sensitive and specific molecular procedure (nested polymerase chain reaction, PCR), using the whole blood sample to establish the diagnosis of acute maternal toxoplasmosis in 27 pregnant women. All of them received the recommended dose of Spiramycin. Only 19 returned for follow-up visits and completed their pregnancies to full term. The achievement of the treatment regimen was evaluated according to the results of PCR amplification of T. gondii DNA at the end of the treatment course. Patients who continued to have positive PCR results were given another treatment course. After treatment with a single course of Spiramycin, 11(57.9%) patients retained T. gondii DNA in their peripheral blood and in eight (42.1%) patients, T. gondii DNA was absent by PCR: four (21.01%) patients received up to three courses of treatment.
弓形虫感染病例的血清学免疫特征具有异质性，反应可能延迟或无法表现出来：这使得它成为一种不可靠的诊断和/或治疗随访方法。因此，本研究依靠一种灵敏且特异的分子程序（巢式聚合酶链反应，PCR），使用全血样本对27名孕妇进行急性母体弓形虫病的诊断。她们都接受了推荐剂量的螺旋霉素。只有19人回来进行随访并足月完成妊娠。根据治疗疗程结束时弓形虫DNA的PCR扩增结果评估治疗方案的成效。PCR结果持续呈阳性的患者接受了另一个疗程的治疗。在接受一个疗程的螺旋霉素治疗后，11名（57.9%）患者外周血中仍保留弓形虫DNA，8名（42.1%）患者的PCR检测未发现弓形虫DNA：4名（21.01%）患者接受了多达三个疗程的治疗。
