存在什么缺陷？利用质量缺陷通过天然质谱法研究纳米盘中介导寡聚化的膜蛋白和肽。
What's the defect? Using mass defects to study oligomerization of membrane proteins and peptides in nanodiscs with native mass spectrometry.
机构信息
Department of Chemistry and Biochemistry and Bio5 Institute, University of Arizona, Tucson, AZ 85721, USA.
Department of Chemistry and Biochemistry and Bio5 Institute, University of Arizona, Tucson, AZ 85721, USA.
出版信息
Methods. 2023 Oct;218:1-13. doi: 10.1016/j.ymeth.2023.07.004. Epub 2023 Jul 22.
Many membrane proteins form functional complexes that are either homo- or hetero-oligomeric. However, it is challenging to characterize membrane protein oligomerization in intact lipid bilayers, especially for polydisperse mixtures. Native mass spectrometry of membrane proteins and peptides inserted in lipid nanodiscs provides a unique method to study the oligomeric state distribution and lipid preferences of oligomeric assemblies. To interpret these complex spectra, we developed novel data analysis methods using macromolecular mass defect analysis. Here, we provide an overview of how mass defect analysis can be used to study oligomerization in nanodiscs, discuss potential limitations in interpretation, and explore strategies to resolve these ambiguities. Finally, we review recent work applying this technique to studying formation of antimicrobial peptide, amyloid protein, and viroporin complexes with lipid membranes.
许多膜蛋白形成功能复合物，这些复合物可以是同型寡聚体或异型寡聚体。然而，在完整的脂质双层中表征膜蛋白寡聚化是具有挑战性的，特别是对于多分散混合物。插入脂质纳米盘的膜蛋白和肽的天然质谱提供了一种独特的方法来研究寡聚体的分布和脂质偏好。为了解释这些复杂的光谱，我们使用大分子质量缺陷分析开发了新的数据分析方法。在这里，我们概述了如何使用质量缺陷分析来研究纳米盘中的寡聚化，讨论了在解释中潜在的限制，并探讨了解决这些模糊性的策略。最后，我们回顾了最近应用该技术研究抗菌肽、淀粉样蛋白和病毒蛋白与脂质膜形成复合物的工作。
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