Saada Helen N, Said Ussama Z, Meky Nefissa H, Abd El Azime Afrag S
Department of Radiation Biology, National Center for Radiation Research and Technology (NCRRT), Atomic Energy Authority, Cairo, Egypt.
Phytother Res. 2009 Mar;23(3):434-8. doi: 10.1002/ptr.2684.
Whole body exposure to ionizing radiation induces the formation of reactive oxygen species (ROS) in different tissues provoking oxidative damage, organ dysfunction and metabolic disturbances. The present study was designed to determine the possible protective effect of grape seed extract (GSE), rich in proanthocyanidins against gamma-radiation-induced oxidative stress in heart and pancreas tissues associated with serum metabolic disturbances. Irradiated rats were whole body exposed to 5 Gy gamma-radiation. GSE-treated irradiated rats received 100 mg GSE/kg/day, by gavage, for 14 days before irradiation. The animals were killed on days 1, 14 and 28 after irradiation. Significant decreases of SOD, CAT and GSH-Px activities associated with significant increases of TBARS levels were recorded in both tissues after irradiation. GSE administration pre-irradiation significantly attenuated the radiation-induced oxidative stress in heart tissues which was substantiated by a significant amelioration of serum LDH, CPK and AST activities. GSE treatment also attenuated the oxidative stress in pancreas tissues which was associated with a significant improvement in radiation-induced hyperglycemia and hyperinsulinemia. In conclusion, the present data demonstrate that GSE would protect the heart and pancreas tissues from oxidative damage induced by ionizing irradiation.
全身暴露于电离辐射会在不同组织中诱导活性氧（ROS）的形成，引发氧化损伤、器官功能障碍和代谢紊乱。本研究旨在确定富含原花青素的葡萄籽提取物（GSE）对与血清代谢紊乱相关的心脏和胰腺组织中γ辐射诱导的氧化应激的可能保护作用。将受辐照大鼠全身暴露于5 Gy的γ辐射。经GSE处理的受辐照大鼠在辐照前14天通过灌胃给予100 mg GSE/kg/天。在辐照后的第1、14和28天处死动物。辐照后，两个组织中均记录到超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和谷胱甘肽过氧化物酶（GSH-Px）活性显著降低，同时丙二醛（TBARS）水平显著升高。辐照前给予GSE可显著减轻心脏组织中辐射诱导的氧化应激，血清乳酸脱氢酶（LDH）、肌酸磷酸激酶（CPK）和天冬氨酸转氨酶（AST）活性的显著改善证实了这一点。GSE处理还减轻了胰腺组织中的氧化应激，这与辐射诱导的高血糖和高胰岛素血症的显著改善相关。总之，目前的数据表明，GSE可保护心脏和胰腺组织免受电离辐射诱导的氧化损伤。
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