Eidi Maryam, Eidi Akram, Saeidi Ali, Molanaei Saadat, Sadeghipour Alireza, Bahar Massih, Bahar Kamal
Department of Biology, Varamin Branch, Islamic Azad University, Varamin, Iran.
Phytother Res. 2009 Mar;23(3):404-6. doi: 10.1002/ptr.2642.
Coriander (Coriandrum sativum L.) is grown as a spice crop all over the world. The seeds have been used to treat indigestion, diabetes, rheumatism and pain in the joints. In the present study, an ethanol extract of the seeds was investigated for effects on insulin release from the pancreatic beta cells in streptozotocin-induced diabetic rats. Blood samples were drawn from the retro-orbital sinus before and 1.5, 3 and 5 h after administration of the seed extract. Serum glucose levels were determined by the glucose oxidase method. To determine the insulin releasing activity, after extract treatment the animals were anaesthetized by diethyl ether, the pancreas was excised, fixed in 10% formaldehyde and embedded in paraffin for sectioning. Pancreatic sections of 5 microm were processed for examination of insulin-releasing activity using an immunocytochemistry kit. The results showed that administration of the ethanol extract (200 and 250 mg/kg, i.p.) exhibited a significant reduction in serum glucose. Administration of streptozotocin decreased the number of beta cells with insulin secretory activity in comparison with intact rats, but treatment with the coriander seed extract (200 mg/kg) increased significantly the activity of the beta cells in comparison with the diabetic control rats. The extract decreased serum glucose in streptozotocin-induced diabetic rats and increased insulin release from the beta cells of the pancreas.
芫荽（Coriandrum sativum L.）作为一种香料作物在全球范围内种植。其种子已被用于治疗消化不良、糖尿病、风湿病和关节疼痛。在本研究中，对芫荽种子的乙醇提取物进行了研究，以探讨其对链脲佐菌素诱导的糖尿病大鼠胰腺β细胞胰岛素释放的影响。在给予种子提取物之前以及给药后1.5、3和5小时，从大鼠眶后窦采集血样。采用葡萄糖氧化酶法测定血清葡萄糖水平。为了确定胰岛素释放活性，提取物处理后，用乙醚麻醉动物，切除胰腺，固定于10%甲醛中，石蜡包埋切片。使用免疫细胞化学试剂盒对5微米厚的胰腺切片进行胰岛素释放活性检测。结果显示，腹腔注射乙醇提取物（200和250毫克/千克）可显著降低血清葡萄糖水平。与正常大鼠相比，链脲佐菌素处理降低了具有胰岛素分泌活性的β细胞数量，但与糖尿病对照大鼠相比，芫荽种子提取物（200毫克/千克）处理显著增加了β细胞的活性。该提取物可降低链脲佐菌素诱导糖尿病大鼠的血清葡萄糖水平，并增加胰腺β细胞的胰岛素释放。
