Gutierrez Rosa Martha Perez, Flores Jose Maria Mota
Laboratory for Research on Natural Products, School of Chemical Engineering and Extractive Industries-IPN. Av. Instituto Politécnico Nacional, Unidad Profesional Adolfo Lopez Mateos CP 07758, Mexico D.F.
Afr J Tradit Complement Altern Med. 2014 Jan 28;11(2):231-6. doi: 10.4314/ajtcam.v11i2.1. eCollection 2014.
In Mexican traditional medicine, the seeds of Byrsonima crassifolia have been used in the treatment of diabetes, rheumatism and for wound healing. The aim was to evaluate the effect of seeds of B. crassifolia on insulin release from the pancreatic beta cells in streptozotocininduced diabetic rats.
In the present study, we evaluated the beneficial effect of seeds B. crassifolia streptozotocin-induced type 1 diabetic rats. Insulin level; insulin sensitivity index, insulin content in pancreas, malonaldehyde, nitric oxide contents, oxidative stress parameters were assayed. Serum glucose levels were determined by the glucose oxidase method. To determine the insulin releasing activity, after extract treatment, the pancreas was excised. Pancreatic sections were processed for examination of insulin-releasing activity using an imunocytochemistry kit.
Administration of the hexane extract (200 and 400 mg/kg), exhibited a significant reduction in serum glucose. Administration of streptozotocin decreased the number of beta cells with insulin secretory activity in comparison with intact rats; but treatment with the B. crassifolia seed extract increased significantly the activity of the beta cells in comparison with the diabetic control rats. The extract decreased serum glucose in streptozotocin-induced diabetic rats and increased insulin release from the beta cells of the pancreas.
These finding suggest that B. crassifolia seed has beneficial effect for diabetes through decreasing blood glucose and lipid levels, increasing insulin sensitivity index and insulin content, up-regulating antioxidant enzyme activity and decreasing lipid peroxidation.
在墨西哥传统医学中，厚叶比蒙木的种子被用于治疗糖尿病、风湿病和伤口愈合。目的是评估厚叶比蒙木种子对链脲佐菌素诱导的糖尿病大鼠胰腺β细胞胰岛素释放的影响。
在本研究中，我们评估了厚叶比蒙木种子对链脲佐菌素诱导的1型糖尿病大鼠的有益作用。检测胰岛素水平、胰岛素敏感性指数、胰腺中的胰岛素含量、丙二醛、一氧化氮含量以及氧化应激参数。采用葡萄糖氧化酶法测定血清葡萄糖水平。为了确定胰岛素释放活性，在提取物处理后，切除胰腺。使用免疫细胞化学试剂盒对胰腺切片进行处理，以检查胰岛素释放活性。
给予己烷提取物（200和400mg/kg）后，血清葡萄糖显著降低。与完整大鼠相比，给予链脲佐菌素后，具有胰岛素分泌活性的β细胞数量减少；但与糖尿病对照大鼠相比，用厚叶比蒙木种子提取物处理后，β细胞的活性显著增加。该提取物降低了链脲佐菌素诱导的糖尿病大鼠的血清葡萄糖水平，并增加了胰腺β细胞的胰岛素释放。
这些发现表明，厚叶比蒙木种子通过降低血糖和血脂水平、增加胰岛素敏感性指数和胰岛素含量、上调抗氧化酶活性以及减少脂质过氧化，对糖尿病具有有益作用。
