Chen Gui An, Cai Xue Yong, Lian Ying, Zheng Xiao Ying, Qiao Jie, Chen Xin Na, Ye Rong Hua
Department of Obstetrics & Gynecology, Third Hospital, Peking University, Peking, China.
Hum Fertil (Camb). 2008 Mar;11(1):49-51. doi: 10.1080/14647270701510025.
To describe the birth achieved from frozen embryos after intracytoplasmic sperm injection (ICSI) of donor sperm into vitrified oocytes.
A 25-year-old woman whose husband was azoospermic undergoing IVF therapy.
Oocytes collected after ovarian stimulation were vitrified, thawed, and fertilized by frozen donor sperm.
Nineteen oocytes were vitrified and all survived after thawing. Thirteen of the 19 oocytes that underwent ICSI with donors sperm were successfully fertilized. Twelve embryos were cryopreserved again by conventional slow-freezing protocol because of uterine bleeding on the day of transfer. Three thawed embryos were transferred, and a normal male with an infant karyotype of 46,XY was delivered.
This case report demonstrates effective oocyte cryopreservation by vitrification.
描述将供体精子通过卵胞浆内单精子注射（ICSI）注入玻璃化冷冻卵母细胞后获得的冻融胚胎的分娩情况。
一名25岁女性，其丈夫无精子症，正在接受体外受精治疗。
经卵巢刺激后采集的卵母细胞进行玻璃化冷冻、解冻，并与冷冻供体精子受精。
19枚卵母细胞进行玻璃化冷冻，解冻后全部存活。19枚接受供体精子ICSI的卵母细胞中有13枚成功受精。由于移植当天子宫出血，12枚胚胎再次采用传统慢速冷冻方案进行冷冻保存。移植了3枚解冻胚胎，分娩出一名核型为46,XY的正常男婴。
本病例报告证明了玻璃化冷冻卵母细胞的有效性。
