Ghosh Susanta Kumar, Tiwari Satyanarayan, Raghavendra Kamaraju, Sathyanarayan Tiruchinapalli Sundaraj, Dash Aditya Prasad
National Institute of Malaria Research, Epidemic Diseases Hospital, Bangalore,India.
J Biosci. 2008 Sep;33(3):333-6. doi: 10.1007/s12038-008-0052-5.
Sporozoites were detected in naturally infected sibling species of the primary rural vector Anopheles culicifacies complex in two primary health centres (PHCs) and a variant of the urban vector Anopheles stephensi in Mangalore city, Karnataka, south India while carrying out malaria outbreak investigations from 1998-2006. Sibling species of An. culicifacies were identified based on the banding patterns on ovarian polytene chromosomes, and variants of An. stephensi were identified based on the number of ridges on the egg floats. Sporozoites were detected in the salivary glands by the dissection method. Of the total 334 salivary glands of An. culicifacies dissected, 17 (5.08%) were found to be positive for sporozoites. Of the 17 positive samples, 11 were suitable for sibling species analysis; 10 were species A (an efficient vector) and 1 was species B (a poor vector). Out of 46 An. stephensi dissected, one was sporozoite positive and belonged to the type form (an efficient vector). In malaria epidemiology this observation is useful for planning an effective vector control programme, because each sibling species/variant differs in host specificity, susceptibility to malarial parasites, breeding habitats and response to insecticides.
在1998年至2006年进行疟疾暴发调查期间，在印度南部卡纳塔克邦芒格洛尔市的两个初级卫生保健中心（PHC）中，在主要农村病媒库蚊复合组自然感染的同胞物种中检测到子孢子，在城市病媒斯氏按蚊的一个变种中也检测到了子孢子。基于卵巢多线染色体上的带型鉴定库蚊的同胞物种，基于卵浮器上的脊数鉴定斯氏按蚊的变种。通过解剖法在唾液腺中检测子孢子。在解剖的总共334个库蚊唾液腺中，有17个（5.08%）被发现子孢子呈阳性。在这17个阳性样本中，有11个适合进行同胞物种分析；其中10个是A种（高效病媒），1个是B种（低效病媒）。在解剖的46只斯氏按蚊中，有一只子孢子呈阳性，属于典型类型（高效病媒）。在疟疾流行病学中，这一观察结果有助于规划有效的病媒控制项目，因为每个同胞物种/变种在宿主特异性、对疟原虫的易感性、繁殖栖息地以及对杀虫剂的反应方面都有所不同。
