Geier Mark S, Smith Cassie L, Butler Ross N, Howarth Gordon S
Centre for Paediatric and Adolescent Gastroenterology, Children, Youth and Women's Health Service, North Adelaide, Adelaide, SA, Australia.
Dig Dis Sci. 2009 Jun;54(6):1222-8. doi: 10.1007/s10620-008-0495-4. Epub 2008 Nov 13.
The dextran sulfate sodium (DSS) colitis model has been utilized to screen for novel therapeutics for ulcerative colitis. Evidence suggests the small intestine may also be affected by DSS. We characterized the effects of DSS on the small intestine and assessed the potential for Lactobacillus fermentum BR11 to modify or normalize DSS-induced changes. Rats were allocated to three groups, Water + Vehicle, DSS + Vehicle, and DSS + L. fermentum BR11. BR11 was administered twice daily for 14 days. DSS (2%) was provided from days 7 to 14. Small-intestinal tissue was analyzed for sucrase activity, histology, and crypt cell proliferation. Increased ileum crypt depth and cell proliferation was observed in DSS-treated rats compared to controls (P < 0.05). BR11 normalized these parameters. While DSS predominantly induces colonic damage, minor morphological alterations were also detected in the distal small intestine. L. fermentum BR11 normalized these features.
硫酸葡聚糖钠（DSS）结肠炎模型已被用于筛选溃疡性结肠炎的新型治疗方法。有证据表明小肠也可能受到DSS的影响。我们对DSS对小肠的影响进行了表征，并评估了发酵乳杆菌BR11改变或使DSS诱导的变化正常化的潜力。将大鼠分为三组，即水+赋形剂组、DSS+赋形剂组和DSS+发酵乳杆菌BR11组。BR11每天给药两次，共14天。从第7天到第14天提供2%的DSS。对小肠组织进行蔗糖酶活性、组织学和隐窝细胞增殖分析。与对照组相比，DSS处理的大鼠回肠隐窝深度和细胞增殖增加（P<0.05）。BR11使这些参数正常化。虽然DSS主要诱导结肠损伤，但在远端小肠也检测到轻微的形态学改变。发酵乳杆菌BR11使这些特征正常化。
