Chen Xiaoyong, Zhao Xin, Wang Hongwei, Yang Zelin, Li Jian, Suo Huayi
College of Food Science, Southwest University, Chongqing 400715, China.
Chongqing Engineering Research Center of Regional Food, Chongqing 400715, China.
Nutrients. 2017 May 25;9(6):545. doi: 10.3390/nu9060545.
The aim of this study is to assess the preventive effects of Lactobacillus fermentum HY01 (LF-HY01) to dextran sulfate sodium induced-colitis. We observed the ratio of colon weight to its length, colon pathological changes, and the concentrations of pro-inflammatory factors (IFN-γ, IL-12, TNFα, and IL-6) in serum. We also took account of the protein levels of IκBα, NF-κB p65, iNOS, and COX-2, and we measured the best effects of different doses of Lactobacillus fermentum HY01 (low dose group was 10⁸ CFU/kg·bw, high dose group was 10 CFU/kg·bw) on dextran sulfate sodium-induced colitis mice. The results were remarkable, suggesting that Lactobacillus fermentum HY01 had significant preventive effects in dextran sulfate sodium induced-colitis; simultaneously, the high dose group showed the best results among other groups. It can effectively alleviate the shortened colon length, reduce the ratio of colon weight to its length, reduce edema, inflammatory cells infiltration, and colon mucosa injury, and play an important role in the down-regulation of concentrations of pro-inflammatory factors (IFN-γ, IL-12, TNFα, and IL-6). Above all, Lactobacillus fermentum HY01 shows promising prevention for IκBα degradation, inhibition of NF-κB p65 phosphorylation cascades, and decreases the protein levels of iNOS and COX-2 as well.
本研究旨在评估发酵乳杆菌HY01（LF-HY01）对葡聚糖硫酸钠诱导的结肠炎的预防作用。我们观察了结肠重量与长度的比值、结肠病理变化以及血清中促炎因子（IFN-γ、IL-12、TNFα和IL-6）的浓度。我们还考虑了IκBα、NF-κB p65、iNOS和COX-2的蛋白水平，并测定了不同剂量的发酵乳杆菌HY01（低剂量组为10⁸ CFU/kg·bw，高剂量组为10¹⁰ CFU/kg·bw）对葡聚糖硫酸钠诱导的结肠炎小鼠的最佳效果。结果显著，表明发酵乳杆菌HY01对葡聚糖硫酸钠诱导的结肠炎具有显著的预防作用；同时，高剂量组在其他组中显示出最佳效果。它可以有效缓解结肠长度缩短，降低结肠重量与长度的比值，减轻水肿、炎症细胞浸润和结肠黏膜损伤，并在下调促炎因子（IFN-γ、IL-12、TNFα和IL-6）浓度方面发挥重要作用。最重要的是，发酵乳杆菌HY01在预防IκBα降解、抑制NF-κB p65磷酸化级联反应以及降低iNOS和COX-2蛋白水平方面也显示出良好的效果。
