Xu Wang Hong, Zheng Wei, Cai Qiuyin, Cheng Jia-Rong, Cai Hui, Xiang Yong-Bing, Shu Xiao Ou
Department of Epidemiology, Cancer Institute of Shanghai Jiao Tong University, Shanghai Cancer Institute, Shanghai, PR China.
Nutr Cancer. 2008;60(6):736-43. doi: 10.1080/01635580802192833.
We evaluated the interactive effect of polymorphisms in the sex hormone-binding globulin (SHBG) gene with soy isoflavones, tea consumption, and dietary fiber on endometrial cancer risk in a population-based, case-control study of 1,199 endometrial cancer patients and 1,212 controls. Genotyping of polymorphisms was performed by using TaqMan (Applied Biosystems, Foster City, CA) assays (rs6259) or the Affymetrix MegAllele Targeted Genotyping System (Affymetrix, Inc., US) (rs13894, rs858521, and rs2955617). Dietary information was obtained using a validated food frequency questionnaire. A logistic regression model was employed to compute adjusted odds ratios (ORs) and 95% confidence intervals (CIs). We found that the Asp(327)Asn (rs6259) polymorphism was associated with decreased risk of endometrial cancer, particularly among postmenopausal women (OR = 0.79, 95% CI = 0.62-1.00). This single nucleotide polymorphism (SNP) modified associations of soy isoflavones and tea consumption but not fiber intake with endometrial cancer, with the inverse association of soy intake and tea consumption being more evident for those with the Asp/Asp genotype of the SHBG gene at Asp(327)Asn (rs6259), particularly premenopausal women (P(interaction) = 0.06 and 0.02, respectively, for soy isoflavones and tea intake). This study suggests that gene-diet interaction may play an important role in the etiology of endometrial cancer risk.
在一项基于人群的病例对照研究中，我们评估了性激素结合球蛋白（SHBG）基因多态性与大豆异黄酮、饮茶及膳食纤维对子宫内膜癌风险的交互作用。该研究纳入了1199例子宫内膜癌患者和1212例对照。采用TaqMan（应用生物系统公司，美国加利福尼亚州福斯特城）分析方法（rs6259）或Affymetrix MegAllele靶向基因分型系统（Affymetrix公司，美国）（rs13894、rs858521和rs2955617）对多态性进行基因分型。通过一份经过验证的食物频率问卷获取饮食信息。采用逻辑回归模型计算调整后的比值比（OR）和95%置信区间（CI）。我们发现，Asp(327)Asn（rs6259）多态性与子宫内膜癌风险降低相关，尤其是在绝经后女性中（OR = 0.79，95% CI = 0.62 - 1.00）。这种单核苷酸多态性（SNP）改变了大豆异黄酮和饮茶与子宫内膜癌的关联，但未改变膳食纤维摄入与子宫内膜癌的关联，对于SHBG基因Asp(327)Asn（rs6259）位点为Asp/Asp基因型的个体，大豆摄入和饮茶与子宫内膜癌的负相关更为明显，尤其是绝经前女性（大豆异黄酮和茶摄入的交互P值分别为0.06和0.02）。本研究表明，基因 - 饮食交互作用可能在子宫内膜癌风险的病因学中起重要作用。
