Ribeiro-Santos Giovanna, Barbisan Luis Fernando, Lopes Fabiane Catanho, Spinardi-Barbisan Ana Lucia Tozzi, da Eira Augusto Ferreira, Kaneno Ramon
Department of Microbiology and Immunology, Institute of Biosciences, Sao Paulo State University-UNESP, Botucatu, SP, Brazil.
Nutr Cancer. 2008;60(6):768-75. doi: 10.1080/01635580802196115.
Chemopreventive and immunomodulatory potential of methanolic (MET) and dichloromethanic (DCl) extracts of Agaricus blazei mushroom were investigated in the postinitiation stage of colon carcinogenesis in male Wistar rats. Animals were initiated with 1,2-dimethylhydrazine (DMH) and treated i.g. with DCl or MET extracts. After 9 wk, animals were sacrificed for evaluation of aberrant crypt foci (ACF) development, crypt cellular proliferation, preneoplastic liver lesions (GST-P), proliferative response of spleen cells to mitogen, and natural killer activity. Administration of DCl extracts did not suppress DMH-induced colonic ACF nor did it affect the crypt multiplicity, but the highest dose of MET significantly reduced the development of preneoplastic lesions in the colon and liver. Lymphoproliferative response was slightly decreased in the initiated control group, which was restored by treatment with MET. No toxicity from DCl and MET extracts was observed (groups MET and DCl).
研究了姬松茸甲醇提取物（MET）和二氯甲烷提取物（DCl）在雄性Wistar大鼠结肠癌发生起始后阶段的化学预防和免疫调节潜力。动物用1,2 - 二甲基肼（DMH）启动，并经口给予DCl或MET提取物。9周后，处死动物以评估异常隐窝灶（ACF）的发展、隐窝细胞增殖、癌前肝损伤（GST - P）、脾细胞对有丝分裂原的增殖反应以及自然杀伤活性。给予DCl提取物并未抑制DMH诱导的结肠ACF，也未影响隐窝的多样性，但最高剂量的MET显著减少了结肠和肝脏癌前病变的发展。起始对照组的淋巴细胞增殖反应略有下降，用MET治疗可使其恢复。未观察到DCl和MET提取物的毒性（MET组和DCl组）。
