Shin Young Sik, Moon Jeong Hee, Kim Myung Soo
Department of Chemistry, Seoul National University, Seoul 151-742, Korea.
Anal Chem. 2008 Dec 15;80(24):9700-4. doi: 10.1021/ac801675r.
A time-of-flight mass spectrometer equipped with two reflectrons was constructed for multiplexed photodissociation tandem mass spectrometry of peptide ions generated by matrix-assisted laser desorption ionization. A linear reflectron was used for high-resolution selection of a precursor ion while a quadratic reflectron was used for product ion analysis. With the photoexcitation of a precursor ion inside a cell floated at high voltage, information (MS(3)) on intermediate ions generating a particular product ion was obtained. Fully multiplexed detection resulted in good MS(3) signal levels. Use of the quadratic reflectron allowed intermediate ion mass determination within 4 Da. The possibility of further extending the technique and its analytical potential are discussed.
构建了一台配备两个反射器的飞行时间质谱仪，用于对基质辅助激光解吸电离产生的肽离子进行多路复用光解离串联质谱分析。线性反射器用于高分辨率选择前体离子，而二次反射器用于产物离子分析。通过对处于高电压下的细胞内的前体离子进行光激发，获得了关于产生特定产物离子的中间离子的信息（MS(3)）。完全多路复用检测产生了良好的MS(3)信号水平。使用二次反射器可在4 Da范围内确定中间离子的质量。讨论了进一步扩展该技术的可能性及其分析潜力。
