Grilló M J, Marín C M, Barberán M, de Miguel M J, Laroucau K, Jacques I, Blasco J M
Instituto de Agrobiotecnología, CSIC-UPNA-Gobierno de Navarra, Carretera de Mutilva, s/n. 31192, Mutilva Baja, Navarra, Spain.
Vaccine. 2009 Jan 7;27(2):187-91. doi: 10.1016/j.vaccine.2008.10.065. Epub 2008 Nov 11.
Brucella melitensis Rev.1 is the most effective vaccine against B. ovis infection in sheep but induces antibodies interfering with B. melitensis diagnosis. Brucella BP26 and Omp31 proteins are differential diagnostic antigens. Single or double bp26 and omp31 Rev.1 deletion mutants have been proven effective against B. melitensis in sheep. Here, the CGV26 (deleted in bp26 gene) and CGV2631 (deleted in both bp26 and omp31 genes) mutants have been tested for efficacy against B. ovis in rams. Either inoculated subcutaneously or conjunctivally, both mutants conferred significant protection against B. ovis. The protection induced by CGV26 was similar to that of Rev.1 but significantly higher than that conferred by CGV2631. In conclusion, the CGV26 mutant, in association with the adequate diagnostic strategy, could be a useful alternative to Rev.1 for sheep vaccination against B. ovis infections in those countries performing simultaneously B. melitensis and B. ovis eradication campaigns.
布鲁氏菌羊种Rev.1是预防绵羊感染绵羊布鲁氏菌最有效的疫苗，但会诱导产生干扰羊种布鲁氏菌诊断的抗体。布鲁氏菌BP26和Omp31蛋白是鉴别诊断抗原。单bp26或双bp26和omp31 Rev.1缺失突变体已被证明对绵羊的羊种布鲁氏菌有效。在此，已对CGV26（bp26基因缺失）和CGV2631（bp26和omp31基因均缺失）突变体对公羊感染绵羊布鲁氏菌的效力进行了测试。无论是皮下接种还是结膜接种，两种突变体都能对绵羊布鲁氏菌提供显著的保护。CGV26诱导的保护作用与Rev.1相似，但显著高于CGV2631。总之，在那些同时开展羊种布鲁氏菌和绵羊布鲁氏菌根除运动的国家，CGV26突变体结合适当的诊断策略，可能是Rev.1用于绵羊预防绵羊布鲁氏菌感染疫苗接种的有用替代品。
