用无乳支原体免疫显性抗原P48进行基因免疫可刺激BALBc小鼠产生混合适应性免疫反应。
Genetic immunization with the immunodominant antigen P48 of Mycoplasma agalactiae stimulates a mixed adaptive immune response in BALBc mice.
作者信息
Chessa Bernardo, Pittau Marco, Puricelli Maria, Zobba Rosanna, Coradduzza Elisabetta, Dall'ara Paola, Rosati Sergio, Poli Giorgio, Alberti Alberto
机构信息
Sezione di Malattie Infettive del Dipartimento di Patologia Speciale e Clinica Veterinaria, Università degli Studi di Sassari, Via Vienna 2, 07100 Sassari, Italy.
出版信息
Res Vet Sci. 2009 Jun;86(3):414-20. doi: 10.1016/j.rvsc.2008.09.010. Epub 2008 Nov 13.
A DNA vaccine against contagious agalactia was developed for the first time, encoding the P48 of Mycoplasma agalactiae. Specific immune responses elicited in BALB/c mice were evaluated. Both total IgG and IgG1 were detected in mice vaccinated with pVAX1/P48. Proliferation of mononuclear cells of the spleen, levels of gamma interferon, interleukin-12, and interleukin-2 mRNAs were enhanced in immunized animals. Results indicate that pVAX1/P48 vaccination induced both T(h)1 and T(h)2 immune responses. Nucleic acid immunization could be a new strategy against M. agalactiae infections and may be potentially used to develop vaccines for other Mycoplasma diseases.
首次研发出一种针对无乳症的DNA疫苗，其编码无乳支原体的P48。评估了在BALB/c小鼠中引发的特异性免疫反应。在用pVAX1/P48疫苗接种的小鼠中检测到了总IgG和IgG1。免疫动物脾脏单核细胞的增殖、γ干扰素、白细胞介素-12和白细胞介素-2 mRNA水平均有所提高。结果表明，pVAX1/P48疫苗接种诱导了Th1和Th2免疫反应。核酸免疫可能是一种对抗无乳支原体感染的新策略，并且可能潜在地用于开发针对其他支原体疾病的疫苗。
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