Sampaio Beatriz Almeida, Barbosa Maysa Santos, de Oliveira Matheus Gonçalves, Santos Júnior Manoel Neres, Guimarães Bruna Carolina de Brito, Andres Emilly Stefane Souza, da Silva Ágatha Morgana Bertoti, Gomes Camila Pacheco, Bittencourt Rafaela de Souza, Correia Thiago Macêdo Lopes, da Silva Lucas Santana Coelho, da Cruz Jurandir Ferreira, Chopra-Dewasthaly Rohini, Campos Guilherme Barreto, Timenetsky Jorge, Bastos Bruno Lopes, Marques Lucas Miranda
Multidisciplinary Institute in Health, Federal University of Bahia, Rua Hormindo Barros 58, Vitória da Conquista 45029-094, BA, Brazil.
Institute of Biomedical Sciences, University of São Paulo, Avenida Professor Lineu Prestes, 2415, Butantã 05508-900, SP, Brazil.
Vaccines (Basel). 2025 Jul 11;13(7):746. doi: 10.3390/vaccines13070746.
: Contagious agalactia (CA) is a disease typically caused by Mycoplasma agalactiae, affecting small ruminants worldwide and being endemic in certain countries. CA causes severe economic losses due to mastitis, agalactia, and arthritis. As an alternative to existing immunoprophylactic measures, this study aimed to develop a recombinant subunit vaccine against and evaluate its specific immune response in goats. : Goats were divided into three groups: group 1 received recombinant proteins (P40 and MAG_1560), group 2 received formalin-inactivated , and group 3 received Tris-buffered saline (negative control). All solutions were emulsified in Freund's adjuvant. Animals were monitored for 181 days. IgG antibody production was assessed by ELISA, and peripheral blood mononuclear cells (PBMCs) were analyzed by real-time PCR for the expression of IL-1β, IFN-γ, IL-12, and MHC class II genes. : -specific antibody response was observed for six months in the sera of animals from group 1. Analysis of cytokine gene expression revealed increased IL-1β mRNA levels over time in both experimental groups. In group 1, IFN-γ mRNA levels increased with P40 stimulation and decreased with MAG_1560. IL-12 mRNA expression decreased over time in group 1 with P40 stimulation, whereas group 2 showed increased IL-12 expression for both proteins. MHC-II expression was stimulated in both groups. : The recombinant proteins induced antibody production and cytokine expression, demonstrating immunogenic potential and supporting their promise as vaccine candidates capable of eliciting both humoral and cellular immune responses against .
传染性无乳症（CA）是一种通常由无乳支原体引起的疾病，影响着全球的小型反刍动物，在某些国家呈地方性流行。CA由于乳腺炎、无乳症和关节炎而导致严重的经济损失。作为现有免疫预防措施的替代方法，本研究旨在开发一种针对[具体病原体未明确写出]的重组亚单位疫苗，并评估其在山羊体内的特异性免疫反应。
第1组接受重组蛋白（P40和MAG_1560），第2组接受福尔马林灭活的[具体病原体未明确写出]，第3组接受三羟甲基氨基甲烷缓冲盐水（阴性对照）。所有溶液均在弗氏佐剂中乳化。对动物进行了181天的监测。通过酶联免疫吸附测定（ELISA）评估IgG抗体产生情况，并通过实时聚合酶链反应（PCR）分析外周血单核细胞（PBMC）中白细胞介素-1β（IL-1β）、干扰素-γ（IFN-γ）、白细胞介素-12（IL-12）和主要组织相容性复合体II类（MHC-II）基因的表达。
在第1组动物的血清中观察到了长达六个月的针对[具体病原体未明确写出]的特异性抗体反应。细胞因子基因表达分析显示，两个实验组中IL-1β信使核糖核酸（mRNA）水平均随时间增加。在第1组中，IFN-γ mRNA水平在P40刺激下升高，在MAG_1560刺激下降低。在第1组中，经P40刺激后，IL-12 mRNA表达随时间下降，而第2组中两种蛋白刺激后IL-12表达均增加。两组中MHC-II表达均受到刺激。
重组蛋白诱导了抗体产生和细胞因子表达，证明了其免疫原性潜力，并支持它们作为能够引发针对[具体病原体未明确写出]的体液免疫和细胞免疫反应的疫苗候选物的前景。
