Takahashi S, Ogawa K, Ohshima H, Esumi H, Ito N, Sugimura T
Biochemistry Division, National Cancer Center Research Institute, Tokyo.
Jpn J Cancer Res. 1991 Feb;82(2):135-7. doi: 10.1111/j.1349-7006.1991.tb01819.x.
Carcinogenicity of 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) to rat colon was investigated using the appearance of colonic aberrant crypt (AC), a preneoplastic lesion, as a marker. The number of AC foci per colon at experimental week 4 was 1.3 +/- 0.8; almost half the level of AC foci induced by 2-amino-6-methyldipyrido[1,2-a:3',2'-d]imidazole (Glu-P-1), which is a known colon carcinogen. No ACs were observed in rats of the control group. A repeat experiment showed that induction of AC foci by PhIP administration was reproducible and a significant increase in the number of AC foci, 3.0 +/- 0.0, was observed after 12 weeks of PhIP administration. The majority of ACs induced by PhIP were localized in the distal part of the colon. The distribution was similar to those induced by Glu-P-1 and 1,2-dimethylhydrazine. Those data suggested that PhIP is possibly carcinogenic to rat colon.
以结肠异常隐窝（AC）这种癌前病变的出现为标志物，研究了2-氨基-1-甲基-6-苯基咪唑并[4,5-b]吡啶（PhIP）对大鼠结肠的致癌性。实验第4周时，每只大鼠结肠的AC病灶数量为1.3±0.8；几乎是已知结肠致癌物2-氨基-6-甲基二吡啶并[1,2-a:3',2'-d]咪唑（Glu-P-1）诱导的AC病灶水平的一半。对照组大鼠未观察到AC。重复实验表明，给予PhIP诱导AC病灶是可重复的，给予PhIP 12周后观察到AC病灶数量显著增加，为3.0±0.0。PhIP诱导的大多数AC位于结肠远端。其分布与Glu-P-1和1,2-二甲基肼诱导的分布相似。这些数据表明，PhIP可能对大鼠结肠具有致癌性。
